CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012274
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tubulin--tyrosine ligase-like protein 12 
Protein Synonyms/Alias
  
Gene Name
 TTLL12 
Gene Synonyms/Alias
 KIAA0153 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
88EAAREVRKQQPNPGNubiquitination[1, 2, 3, 4]
100PGNELCYKVIVTRESubiquitination[3]
187AHGTAEEKMPVWYIMubiquitination[1]
253ETDPLIRKCMLLPWAubiquitination[3]
342LFNFSHFKDYRKLSQubiquitination[1, 3, 5, 6]
346SHFKDYRKLSQERPGubiquitination[3]
368CENLLTVKDCLASIAubiquitination[1, 3, 6]
419EDNHWICKPWNLARSubiquitination[1, 3]
433SLDTHVTKSLHSIIRubiquitination[1, 3, 4, 5, 7, 8]
451STPKVVSKYIESPVLubiquitination[3, 5]
510NDLDDYEKHFTVMNYacetylation[9]
524YDPDVVLKQVHCEEFubiquitination[1]
565LFQVACAKPPPLGLCubiquitination[1, 3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224
Functional Description
  
Sequence Annotation
 DOMAIN 300 644 TTL.
 MOD_RES 16 16 Phosphoserine (By similarity).  
Keyword
 Complete proteome; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 644 AA 
Protein Sequence
MEAERGPERR PAERSSPGQT PEEGAQALAE FAALHGPALR ASGVPERYWG RLLHKLEHEV 60
FDAGEVFGIM QVEEVEEEED EAAREVRKQQ PNPGNELCYK VIVTRESGLQ AAHPNSIFLI 120
DHAWTCRVEH ARQQLQQVPG LLHRMANLMG IEFHGELPST EAVALVLEEM WKFNQTYQLA 180
HGTAEEKMPV WYIMDEFGSR IQHADVPSFA TAPFFYMPQQ VAYTLLWPLR DLDTGEEVTR 240
DFAYGETDPL IRKCMLLPWA PTDMLDLSSC TPEPPAEHYQ AILEENKEKL PLDINPVVHP 300
HGHIFKVYTD VQQVASSLTH PRFTLTQSEA DADILFNFSH FKDYRKLSQE RPGVLLNQFP 360
CENLLTVKDC LASIARRAGG PEGPPWLPRT FNLRTELPQF VSYFQQRERW GEDNHWICKP 420
WNLARSLDTH VTKSLHSIIR HRESTPKVVS KYIESPVLFL REDVGKVKFD IRYIVLLRSV 480
RPLRLFVYDV FWLRFSNRAF ALNDLDDYEK HFTVMNYDPD VVLKQVHCEE FIPEFEKQYP 540
EFPWTDVQAE IFRAFTELFQ VACAKPPPLG LCDYPSSRAM YAVDLMLKWD NGPDGRRVMQ 600
PQILEVNFNP DCERACRYHP TFFNDVFSTL FLDQPGGCHV TCLV 644 
Gene Ontology
 GO:0004835; F:tubulin-tyrosine ligase activity; IEA:InterPro.
 GO:0006464; P:cellular protein modification process; IEA:InterPro. 
Interpro
 IPR004344; Tub_tyr_ligase. 
Pfam
 PF03133; TTL 
SMART
  
PROSITE
 PS51221; TTL 
PRINTS