UniProt Accession

 H2AY_HUMAN; O75367; O75377; Q503A8; Q7Z5E3; Q96D41; Q9H8P3; Q9UP96 

Genbank Protein ID

 AF041483; AF044286; AF054174; AK023409; AC026691; BC013331; BC095406; AY134746 

Genbank Nucleotide ID

 AAC33433.1; AAC33434.1; AAC39908.1; BAB14565.1; AAH13331.1; AAH95406.1; AAN08620.1 

Protein Name

 Core histone macro-H2A.1 

Protein Synonyms/Alias

 Histone macroH2A1; mH2A1; Histone H2A.y; H2A/y; Medulloblastoma antigen MU-MB-50.205 

Gene Name

 H2AFY 

Gene Synonyms/Alias

 MACROH2A1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
18	TKTSRSAKAGVIFPV	ubiquitination	[1]
116	IHPELLAKKRGSKGK	ubiquitination	[1]
117	HPELLAKKRGSKGKL	ubiquitination	[1]
123	KKRGSKGKLEAIITP	ubiquitination	[1]
167	KKQGEVSKAASADST	ubiquitination	[1]
189	GFTVLSTKSLFLGQK	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. 

Sequence Annotation

 DOMAIN 2 117 Histone H2A.
 DOMAIN 184 370 Macro.
 MOD_RES 18 18 N6-methyllysine.
 MOD_RES 123 123 N6,N6-dimethyllysine (Probable).
 MOD_RES 129 129 Phosphothreonine.
 MOD_RES 170 170 Phosphoserine.
 MOD_RES 173 173 Phosphoserine.
 MOD_RES 178 178 Phosphothreonine.
 CROSSLNK 116 116 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 117 117 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Alternative splicing; Chromatin regulator; Chromosome; Complete proteome; DNA-binding; Isopeptide bond; Methylation; Nucleosome core; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 372 AA 

Protein Sequence

Gene Ontology

 GO:0001740; C:Barr body; IDA:MGI.
 GO:0000793; C:condensed chromosome; IEA:Compara.
 GO:0000786; C:nucleosome; NAS:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; NAS:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0007549; P:dosage compensation; IDA:MGI.
 GO:0006334; P:nucleosome assembly; NAS:UniProtKB. 

Interpro

 IPR002589; A1pp.
 IPR021171; Core_histone_macro-H2A.
 IPR009072; Histone-fold.
 IPR007125; Histone_core_D.
 IPR002119; Histone_H2A. 

Pfam

 PF00125; Histone
 PF01661; Macro 

SMART

 SM00506; A1pp
 SM00414; H2A 

PROSITE

 PS51154; MACRO 

PRINTS

 PR00620; HISTONEH2A.