CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006685
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal RNA small subunit methyltransferase B 
Protein Synonyms/Alias
 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RsmB 
Gene Name
 rsmB 
Gene Synonyms/Alias
 fmu/fmv; rrmB; sun; yhdB; b3289; JW3250 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
151LHPSWLLKRLQKAYPacetylation[1]
425GDGFFYAKLIKK***acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA. 
Sequence Annotation
 REGION 254 260 S-adenosyl-L-methionine binding.
 ACT_SITE 375 375 Nucleophile (Potential).
 BINDING 277 277 S-adenosyl-L-methionine.
 BINDING 303 303 S-adenosyl-L-methionine.
 BINDING 322 322 S-adenosyl-L-methionine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Reference proteome; RNA-binding; rRNA processing; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 429 AA 
Protein Sequence
MKKQRNLRSM AAQAVEQVVE QGQSLSNILP PLQQKVSDKD KALLQELCFG VLRTLSQLDW 60
LINKLMARPM TGKQRTVHYL IMVGLYQLLY TRIPPHAALA ETVEGAIAIK RPQLKGLING 120
VLRQFQRQQE ELLAEFNASD ARYLHPSWLL KRLQKAYPEQ WQSIVEANNQ RPPMWLRINR 180
THHSRDSWLA LLDEAGMKGF PHADYPDAVR LETPAPVHAL PGFEDGWVTV QDASAQGCMT 240
WLAPQNGEHI LDLCAAPGGK TTHILEVAPE AQVVAVDIDE QRLSRVYDNL KRLGMKATVK 300
QGDGRYPSQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIPELA QLQSEILDAI 360
WPHLKTGGTL VYATCSVLPE ENSLQIKAFL QRTADAELCE TGTPEQPGKQ NLPGAEEGDG 420
FFYAKLIKK 429 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0009383; F:rRNA (cytosine-C5-)-methyltransferase activity; IDA:EcoCyc.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0070475; P:rRNA base methylation; IMP:EcoCyc. 
Interpro
 IPR001678; Fmu/NOL1/Nop2p.
 IPR018314; Fmu/NOL1/Nop2p_CS.
 IPR006027; NusB_RsmB_TIM44.
 IPR023267; RCMT.
 IPR004573; rRNA_ssu_MeTfrase_B.
 IPR023541; rRNA_ssu_MeTfrase_B_ent. 
Pfam
 PF01189; Nol1_Nop2_Fmu
 PF01029; NusB 
SMART
  
PROSITE
 PS01153; NOL1_NOP2_SUN 
PRINTS
 PR02008; RCMTFAMILY.