CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007356
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nucleoporin NUP159 
Protein Synonyms/Alias
 Nuclear pore protein NUP159 
Gene Name
 NUP159 
Gene Synonyms/Alias
 NUP158; RAT7; YIL115C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
5***MSSLKDEVPTETacetylation[1]
897PMEEADTKETSEKGPubiquitination[2]
1024ALKKDNEKENFDSNMubiquitination[3]
1292NANIQDLKEKVTDYVubiquitination[3]
Reference
 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [2] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export. 
Sequence Annotation
 REPEAT 228 231 FG 1.
 REPEAT 267 270 PXFG 1.
 REPEAT 462 470 SXFGXPXFG 1.
 REPEAT 503 511 SXFGXPXFG 2; approximate.
 REPEAT 522 530 SXFGXPXFG 3; approximate.
 REPEAT 532 535 PXFG 2.
 REPEAT 548 556 SXFGXPXFG 4.
 REPEAT 558 561 PXFG 3.
 REPEAT 574 582 SXFGXPXFG 5.
 REPEAT 584 587 PXFG 4.
 REPEAT 600 608 SXFGXPXFG 6.
 REPEAT 610 613 SXFG 1.
 REPEAT 624 632 SXFGXPXFG 7; approximate.
 REPEAT 642 645 FG 2.
 REPEAT 687 690 FG 3.
 REPEAT 704 707 FXFG 1.
 REPEAT 709 712 SXFG 2.
 REPEAT 728 731 FXFG 2.
 REPEAT 842 845 PXFG 5.
 REPEAT 873 876 FXFG 3.
 REGION 1 500 Interaction with DBP5.
 REGION 497 701 Interactions with CRM1 and GLE1.
 REGION 1223 1460 Interaction with NUP82.
 MOD_RES 404 404 Phosphoserine.
 MOD_RES 657 657 Phosphoserine.
 MOD_RES 724 724 Phosphoserine.
 MOD_RES 735 735 Phosphoserine.
 MOD_RES 745 745 Phosphoserine.
 MOD_RES 803 803 Phosphothreonine.
 MOD_RES 805 805 Phosphoserine.
 MOD_RES 819 819 Phosphoserine.
 MOD_RES 889 889 Phosphoserine.
 MOD_RES 940 940 Phosphoserine.  
Keyword
 3D-structure; Coiled coil; Complete proteome; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1460 AA 
Protein Sequence
MSSLKDEVPT ETSEDFGFKF LGQKQILPSF NEKLPFASLQ NLDISNSKSL FVAASGSKAV 60
VGELQLLRDH ITSDSTPLTF KWEKEIPDVI FVCFHGDQVL VSTRNALYSL DLEELSEFRT 120
VTSFEKPVFQ LKNVNNTLVI LNSVNDLSAL DLRTKSTKQL AQNVTSFDVT NSQLAVLLKD 180
RSFQSFAWRN GEMEKQFEFS LPSELEELPV EEYSPLSVTI LSPQDFLAVF GNVISETDDE 240
VSYDQKMYII KHIDGSASFQ ETFDITPPFG QIVRFPYMYK VTLSGLIEPD ANVNVLASSC 300
SSEVSIWDSK QVIEPSQDSE RAVLPISEET DKDTNPIGVA VDVVTSGTIL EPCSGVDTIE 360
RLPLVYILNN EGSLQIVGLF HVAAIKSGHY SINLESLEHE KSLSPTSEKI PIAGQEQEEK 420
KKNNESSKAL SENPFTSANT SGFTFLKTQP AAANSLQSQS SSTFGAPSFG SSAFKIDLPS 480
VSSTSTGVAS SEQDATDPAS AKPVFGKPAF GAIAKEPSTS EYAFGKPSFG APSFGSGKSS 540
VESPASGSAF GKPSFGTPSF GSGNSSVEPP ASGSAFGKPS FGTPSFGSGN SSAEPPASGS 600
AFGKPSFGTS AFGTASSNET NSGSIFGKAA FGSSSFAPAN NELFGSNFTI SKPTVDSPKE 660
VDSTSPFPSS GDQSEDESKS DVDSSSTPFG TKPNTSTKPK TNAFDFGSSS FGSGFSKALE 720
SVGSDTTFKF GTQASPFSSQ LGNKSPFSSF TKDDTENGSL SKGSTSEIND DNEEHESNGP 780
NVSGNDLTDS TVEQTSSTRL PETPSDEDGE VVEEEAQKSP IGKLTETIKK SANIDMAGLK 840
NPVFGNHVKA KSESPFSAFA TNITKPSSTT PAFSFGNSTM NKSNTSTVSP MEEADTKETS 900
EKGPITLKSV ENPFLPAKEE RTGESSKKDH NDDPKDGYVS GSEISVRTSE SAFDTTANEE 960
IPKSQDVNNH EKSETDPKYS QHAVVDHDNK SKEMNETSKN NERSGQPNHG VQGDGIALKK 1020
DNEKENFDSN MAIKQFEDHQ SSEEDASEKD SRQSSEVKES DDNMSLNSDR DESISESYDK 1080
LEDINTDELP HGGEAFKARE VSASADFDVQ TSLEDNYAES GIQTDLSESS KENEVQTDAI 1140
PVKHNSTQTV KKEAVDNGLQ TEPVETCNFS VQTFEGDENY LAEQCKPKQL KEYYTSAKVS 1200
NIPFVSQNST LRLIESTFQT VEAEFTVLME NIRNMDTFFT DQSSIPLVKR TVRSINNLYT 1260
WRIPEAEILL NIQNNIKCEQ MQITNANIQD LKEKVTDYVR KDIAQITEDV ANAKEEYLFL 1320
MHFDDASSGY VKDLSTHQFR MQKTLRQKLF DVSAKINHTE ELLNILKLFT VKNKRLDDNP 1380
LVAKLAKESL ARDGLLKEIK LLREQVSRLQ LEEKGKKASS FDASSSITKD MKGFKVVEVG 1440
LAMNTKKQIG DFFKNLNMAK 1460 
Gene Ontology
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0044613; C:nuclear pore central transport channel; IDA:SGD.
 GO:0044614; C:nuclear pore cytoplasmic filaments; IDA:SGD.
 GO:0000774; F:adenyl-nucleotide exchange factor activity; IMP:SGD.
 GO:0005487; F:nucleocytoplasmic transporter activity; IPI:SGD.
 GO:0097064; P:ncRNA export from nucleus; IMP:SGD.
 GO:0006607; P:NLS-bearing substrate import into nucleus; IGI:SGD.
 GO:0031081; P:nuclear pore distribution; IMP:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
 GO:0006611; P:protein export from nucleus; IMP:SGD.
 GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD.
 GO:0000056; P:ribosomal small subunit export from nucleus; IMP:SGD. 
Interpro
 IPR015943; WD40/YVTN_repeat-like_dom. 
Pfam
  
SMART
  
PROSITE
  
PRINTS