CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011796
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA pseudouridine synthase 1 
Protein Synonyms/Alias
 tRNA pseudouridylate synthase 1; tRNA-uridine isomerase 1 
Gene Name
 PUS1 
Gene Synonyms/Alias
 YPL212C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
20QVNEDVYKRGAQSKLacetylation[1]
156IIEDPDIKQKINEKLubiquitination[2]
394QQKINIPKAPALGLLubiquitination[2]
442KMKHIYDKIYKEEVDacetylation[1]
477QGEETADKSGPAVQKubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). 
Sequence Annotation
 ACT_SITE 134 134 Nucleophile (By similarity).
 BINDING 190 190 Substrate (By similarity).  
Keyword
 Complete proteome; Isomerase; Nucleus; Reference proteome; tRNA processing; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 544 AA 
Protein Sequence
MSEENLRPAY DDQVNEDVYK RGAQSKLTKA RKADFDDEKD KKKDNDKHID KRPKSGPRLD 60
ENGNPLPKEP RLPKRKVAVM VGYCGTGYHG MQYNPPNPTI ESALFKAFVE AGAISKDNSN 120
DLKKNGFMRA ARTDKGVHAG GNLISLKMII EDPDIKQKIN EKLPEGIRVW DIERVNKAFD 180
CRKMCSSRWY EYLLPTYSLI GPKPGSILYR DIEESKTELP GVLDEDLESK EFWEEFKKDA 240
NEKFSTEEIE AILAYVPPAR DEFDINEELY QKVKKYKQLE NAHRRRYRIS AAKLAKFRAS 300
TSQYLGAHNF HNFTLGKDFK EPSAIRFMKD IKVSDPFVIG DAQTEWISIK IHGQSFMLHQ 360
IRKMVSMATL ITRCGCPVER ISQAYGQQKI NIPKAPALGL LLEAPVFEGY NKRLEQFGYK 420
AIDFSKYQDE VDKFKMKHIY DKIYKEEVDE NVFNAFFSYI DSFNKVTGAQ GEETADKSGP 480
AVQKSIFEFL TAKGIPGLTD APESNKKIKQ RKRMEEEEAA SKKAEISSTT QSNEPEVQPE 540
AAAN 544 
Gene Ontology
 GO:0005634; C:nucleus; IPI:SGD.
 GO:0009982; F:pseudouridine synthase activity; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0031120; P:snRNA pseudouridine synthesis; IMP:SGD.
 GO:0031119; P:tRNA pseudouridine synthesis; IDA:SGD. 
Interpro
 IPR020103; PsdUridine_synth_cat_dom.
 IPR001406; PsdUridine_synth_TruA.
 IPR020097; PsdUridine_synth_TruA_a/b_dom.
 IPR020095; PsdUridine_synth_TruA_C.
 IPR020094; PsdUridine_synth_TruA_N. 
Pfam
 PF01416; PseudoU_synth_1 
SMART
  
PROSITE
  
PRINTS