UniProt Accession

 DMA_ECOLI; P0AEE8; P00475; Q2M752 

Genbank Protein ID

 J01600; V00272; Z19601; U18997; U00096; AP009048; X15162 

Genbank Nucleotide ID

 AAA23664.1; CAA23530.1; CAA79668.1; AAA58184.1; AAC76412.1; BAE77904.1; CAA33254.1 

Protein Name

 DNA adenine methylase 

Protein Synonyms/Alias

 DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.EcoDam 

Gene Name

 dam 

Gene Synonyms/Alias

 b3387; JW3350 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
22	YPLLDDIKRHLPKGE	acetylation	[1]
100	QFREEFNKSQDPFRR	acetylation	[1]
140	VPFGRYKKPYFPEAE	acetylation	[1]
259	SSNGGTRKKVDELLA	acetylation	[1]
269	DELLALYKPGVVSPA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication. 

Sequence Annotation

 BINDING 10 10 S-adenosyl-L-methionine (By similarity).
 BINDING 14 14 S-adenosyl-L-methionine; via amide
 BINDING 54 54 S-adenosyl-L-methionine (By similarity).
 BINDING 181 181 S-adenosyl-L-methionine (By similarity).  

Keyword

 3D-structure; Complete proteome; DNA replication; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 278 AA 

Protein Sequence

Gene Ontology

 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IDA:EcoliWiki.
 GO:0006261; P:DNA-dependent DNA replication; IMP:EcoliWiki.
 GO:0006298; P:mismatch repair; IDA:EcoliWiki. 

Interpro

 IPR023095; Ade_MeTrfase_dom_2.
 IPR002052; DNA_methylase_N6_adenine_CS.
 IPR012263; M_m6A_EcoRV.
 IPR012327; MeTrfase_D12. 

Pfam

 PF02086; MethyltransfD12 

SMART

  

PROSITE

 PS00092; N6_MTASE 

PRINTS

 PR00505; D12N6MTFRASE.