UniProt Accession

 H12_MOUSE; P15864 

Genbank Protein ID

 M25365; J03482; Y12291; AY158905 

Genbank Nucleotide ID

 AAA37808.1; AAA37807.1; CAA72970.1; AAO06216.1 

Protein Name

 Histone H1.2 

Protein Synonyms/Alias

 H1 VAR.1; H1c 

Gene Name

 Hist1h1c 

Gene Synonyms/Alias

 H1f2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
17	AAAPPAEKAPAKKKA	acetylation	[1, 2, 3, 4]
17	AAAPPAEKAPAKKKA	methylation	[2]
17	AAAPPAEKAPAKKKA	succinylation	[4]
17	AAAPPAEKAPAKKKA	ubiquitination	[5]
22	AEKAPAKKKAAKKPA	methylation	[2]
23	EKAPAKKKAAKKPAG	acetylation	[6]
26	PAKKKAAKKPAGVRR	methylation	[2]
26	PAKKKAAKKPAGVRR	ubiquitination	[5]
27	AKKKAAKKPAGVRRK	ubiquitination	[5]
34	KPAGVRRKASGPPVS	acetylation	[4]
34	KPAGVRRKASGPPVS	crotonylation	[7]
34	KPAGVRRKASGPPVS	methylation	[2]
34	KPAGVRRKASGPPVS	succinylation	[4]
34	KPAGVRRKASGPPVS	ubiquitination	[8]
46	PVSELITKAVAASKE	acetylation	[2, 3, 4, 9, 10]
46	PVSELITKAVAASKE	succinylation	[4]
46	PVSELITKAVAASKE	ubiquitination	[8]
52	TKAVAASKERSGVSL	acetylation	[1, 2]
52	TKAVAASKERSGVSL	crotonylation	[2]
52	TKAVAASKERSGVSL	methylation	[2]
63	GVSLAALKKALAAAG	acetylation	[3, 4]
63	GVSLAALKKALAAAG	ubiquitination	[8]
64	VSLAALKKALAAAGY	crotonylation	[7]
85	SRIKLGLKSLVSKGI	acetylation	[1, 3, 4, 10]
85	SRIKLGLKSLVSKGI	crotonylation	[7]
85	SRIKLGLKSLVSKGI	ubiquitination	[8]
90	GLKSLVSKGILVQTK	acetylation	[1, 3, 4]
90	GLKSLVSKGILVQTK	ubiquitination	[8]
97	KGILVQTKGTGASGS	acetylation	[3, 4]
97	KGILVQTKGTGASGS	methylation	[2]
97	KGILVQTKGTGASGS	succinylation	[4]
97	KGILVQTKGTGASGS	ubiquitination	[8]
106	TGASGSFKLNKKAAS	acetylation	[3, 4]
106	TGASGSFKLNKKAAS	succinylation	[4]
106	TGASGSFKLNKKAAS	ubiquitination	[8]
110	GSFKLNKKAASGEAK	methylation	[2]
121	GEAKPQAKKAGAAKA	acetylation	[4]
121	GEAKPQAKKAGAAKA	succinylation	[4]
127	AKKAGAAKAKKPAGA	acetylation	[6]
136	KKPAGAAKKPKKATG	acetylation	[6]
137	KPAGAAKKPKKATGA	acetylation	[6]
159	KKTPKKAKKPAAAAV	acetylation	[11]
159	KKTPKKAKKPAAAAV	crotonylation	[7]
160	KTPKKAKKPAAAAVT	acetylation	[1]
160	KTPKKAKKPAAAAVT	ubiquitination	[5]
168	PAAAAVTKKVAKSPK	acetylation	[1, 2, 4, 11]
168	PAAAAVTKKVAKSPK	crotonylation	[7]
175	KKVAKSPKKAKVTKP	acetylation	[3]
176	KVAKSPKKAKVTKPK	acetylation	[3]
178	AKSPKKAKVTKPKKV	acetylation	[1, 3]

Reference

 [1] Mass spectrometric mapping of linker histone H1 variants reveals multiple acetylations, methylations, and phosphorylation as well as differences between cell culture and tissue.
 Wisniewski JR, Zougman A, Krüger S, Mann M.
 Mol Cell Proteomics. 2007 Jan;6(1):72-87. [PMID: 17043054]
 [2] Identification of combinatorial patterns of post-translational modifications on individual histones in the mouse brain.
 Tweedie-Cullen RY, Brunner AM, Grossmann J, Mohanna S, Sichau D, Nanni P, Panse C, Mansuy IM.
 PLoS One. 2012;7(5):e36980. [PMID: 22693562]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [5] BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs.
 Mathew R, Seiler MP, Scanlon ST, Mao AP, Constantinides MG, Bertozzi-Villa C, Singer JD, Bendelac A.
 Nature. 2012 Nov 22;491(7425):618-21. [PMID: 23086144]
 [6] Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain.
 Tweedie-Cullen RY, Reck JM, Mansuy IM.
 J Proteome Res. 2009 Nov;8(11):4966-82. [PMID: 19737024]
 [7] Identification of 67 histone marks and histone lysine crotonylation as a new type of histone modification.
 Tan M, Luo H, Lee S, Jin F, Yang JS, Montellier E, Buchou T, Cheng Z, Rousseaux S, Rajagopal N, Lu Z, Ye Z, Zhu Q, Wysocka J, Ye Y, Khochbin S, Ren B, Zhao Y.
 Cell. 2011 Sep 16;146(6):1016-28. [PMID: 21925322]
 [8] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [9] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [11] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). 

Sequence Annotation

 DOMAIN 36 109 H15.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 2 2 Phosphoserine (By similarity).
 MOD_RES 34 34 N6-crotonyl-L-lysine; alternate.
 MOD_RES 34 34 N6-methyllysine; alternate (By
 MOD_RES 64 64 N6-crotonyl-L-lysine.
 MOD_RES 85 85 N6-crotonyl-L-lysine.
 MOD_RES 90 90 N6-crotonyl-L-lysine (By similarity).
 MOD_RES 97 97 N6-crotonyl-L-lysine (By similarity).
 MOD_RES 104 104 Phosphoserine; by PKC (By similarity).
 MOD_RES 146 146 Phosphothreonine (By similarity).
 MOD_RES 159 159 N6-crotonyl-L-lysine.
 MOD_RES 168 168 N6-crotonyl-L-lysine.
 MOD_RES 186 186 N6-methyllysine; by EHMT1 and EHMT2 (By
 CROSSLNK 17 17 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 205 205 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Chromosome; Complete proteome; DNA-binding; Isopeptide bond; Methylation; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 212 AA 

Protein Sequence

Gene Ontology

 GO:0000786; C:nucleosome; IEA:InterPro.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IDA:MGI.
 GO:0006334; P:nucleosome assembly; IEA:InterPro.
 GO:0016584; P:nucleosome positioning; IMP:MGI. 

Interpro

 IPR005818; Histone_H1/H5.
 IPR005819; Histone_H5.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00538; Linker_histone 

SMART

 SM00526; H15 

PROSITE

 PS51504; H15 

PRINTS

 PR00624; HISTONEH5.