CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009219
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 GMP reductase 
Protein Synonyms/Alias
 Guanosine 5'-monophosphate oxidoreductase; Guanosine monophosphate reductase 
Gene Name
 guaC 
Gene Synonyms/Alias
 b0104; JW0101 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
12EDLKLGFKDVLIRPKacetylation[1]
112TSDADFEKTKQILDLacetylation[1]
276MSSESAMKRHVGGVAacetylation[1]
294AAEGKTVKLPLRGPVacetylation[1, 2]
325YVGASRLKELTKRTTacetylation[1]
329SRLKELTKRTTFIRVacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 
Functional Description
 Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides. 
Sequence Annotation
 NP_BIND 108 131 NADP (By similarity).
 NP_BIND 216 239 NADP (By similarity).
 ACT_SITE 186 186 Thioimidate intermediate (By similarity).
 METAL 181 181 Potassium; via carbonyl oxygen (By
 METAL 183 183 Potassium; via carbonyl oxygen (By  
Keyword
 Complete proteome; Direct protein sequencing; Metal-binding; NADP; Oxidoreductase; Potassium; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 347 AA 
Protein Sequence
MRIEEDLKLG FKDVLIRPKR STLKSRSDVE LERQFTFKHS GQSWSGVPII AANMDTVGTF 60
SMASALASFD ILTAVHKHYS VEEWQAFINN SSADVLKHVM VSTGTSDADF EKTKQILDLN 120
PALNFVCIDV ANGYSEHFVQ FVAKAREAWP TKTICAGNVV TGEMCEELIL SGADIVKVGI 180
GPGSVCTTRV KTGVGYPQLS AVIECADAAH GLGGMIVSDG GCTTPGDVAK AFGGGADFVM 240
LGGMLAGHEE SGGRIVEENG EKFMLFYGMS SESAMKRHVG GVAEYRAAEG KTVKLPLRGP 300
VENTARDILG GLRSACTYVG ASRLKELTKR TTFIRVQEQE NRIFNNL 347 
Gene Ontology
 GO:0005737; C:cytoplasm; IC:EcoCyc.
 GO:0003920; F:GMP reductase activity; IDA:EcoCyc.
 GO:0042802; F:identical protein binding; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006163; P:purine nucleotide metabolic process; IEA:HAMAP. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR005993; GMP_reduct1.
 IPR015875; IMP_DH/GMP_Rdtase_CS.
 IPR001093; IMP_DH_GMPRt. 
Pfam
 PF00478; IMPDH 
SMART
  
PROSITE
 PS00487; IMP_DH_GMP_RED 
PRINTS