UniProt Accession

 MTU1_HUMAN; O75648; A8K3U7; Q05C99; Q5W9C8; Q66K31; Q6ICC3; Q9NWC1 

Genbank Protein ID

 AY062123; AF448221; AB178028; CR456445; AK001002; AK290712; AL031588; AL031588; CH471138; CH471138; BC027991; BC080631 

Genbank Nucleotide ID

 AAL38183.1; AAL35970.1; BAD66875.1; CAG30331.1; BAA91462.1; BAF83401.1; CAB38414.1; CAP58845.1; EAW73422.1; EAW73426.1; AAH27991.1; AAH80631.1 

Protein Name

 Mitochondrial tRNA-specific 2-thiouridylase 1 

Protein Synonyms/Alias

 MTO2 homolog 

Gene Name

 TRMU 

Gene Synonyms/Alias

 MTU1; TRMT1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
286	VVEKDSVKGDVFVAP	ubiquitination	[1]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2- thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base. 

Sequence Annotation

 NP_BIND 10 17 ATP (By similarity).
 REGION 96 98 Interaction with target base in tRNA (By
 REGION 171 173 Interaction with tRNA (By similarity).
 REGION 334 335 Interaction with tRNA (By similarity).
 ACT_SITE 101 101 Nucleophile (By similarity).
 ACT_SITE 222 222 Cysteine persulfide intermediate (By
 BINDING 36 36 ATP; via amide nitrogen and carbonyl
 BINDING 126 126 ATP; via amide nitrogen (By similarity).
 DISULFID 101 222 Alternate (By similarity).  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Disease mutation; Disulfide bond; Mitochondrion; Nucleotide-binding; Polymorphism; Reference proteome; RNA-binding; Transferase; tRNA processing; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 421 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
 GO:0004808; F:tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity; NAS:UniProtKB.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW. 

Interpro

 IPR023382; Adenine_a_hdrlase_dom.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR004506; tRNA-specific_2-thiouridylase. 

Pfam

 PF03054; tRNA_Me_trans 

SMART

  

PROSITE

  

PRINTS