| Tag | Content |
|---|
| CPLM ID | CPLM-006767 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Isoaspartyl peptidase |
Protein Synonyms/Alias | Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase; Isoaspartyl peptidase subunit alpha; Isoaspartyl peptidase subunit beta |
Gene Name | iaaA |
Gene Synonyms/Alias | spt; ybiK; b0828; JW0812 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 174 | SGAPLDEKQKMGTVG | acetylation | [1] | | 314 | TTGIYREKGDTVATQ | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Degrades L- isoaspartyl-containing di- and maybe also tripeptides. Also has L- asparaginase activity, although this may not be its principal function. |
Sequence Annotation | REGION 207 210 Substrate binding.
REGION 230 233 Substrate binding.
ACT_SITE 179 179 Nucleophile. |
Keyword | 3D-structure; Autocatalytic cleavage; Complete proteome; Direct protein sequencing; Hydrolase; Protease; Reference proteome. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 321 AA |
Protein Sequence | MGKAVIAIHG GAGAISRAQM SLQQELRYIE ALSAIVETGQ KMLEAGESAL DVVTEAVRLL 60
EECPLFNAGI GAVFTRDETH ELDACVMDGN TLKAGAVAGV SHLRNPVLAA RLVMEQSPHV 120
MMIGEGAENF AFARGMERVS PEIFSTSLRY EQLLAARKEG ATVLDHSGAP LDEKQKMGTV 180
GAVALDLDGN LAAATSTGGM TNKLPGRVGD SPLVGAGCYA NNASVAVSCT GTGEVFIRAL 240
AAYDIAALMD YGGLSLAEAC ERVVMEKLPA LGGSGGLIAI DHEGNVALPF NTEGMYRAWG 300
YAGDTPTTGI YREKGDTVAT Q 321 |
Gene Ontology | GO:0004067; F:asparaginase activity; IDA:EcoCyc. GO:0008798; F:beta-aspartyl-peptidase activity; IDA:EcoCyc. GO:0016540; P:protein autoprocessing; IMP:EcoCyc. GO:0006508; P:proteolysis; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |