UniProt Accession

 TNK1_HUMAN; Q13470; O95364; Q8IYI4 

Genbank Protein ID

 U43408; AF097738; BC035782 

Genbank Nucleotide ID

 AAC50427.1; AAC99412.1; AAH35782.1 

Protein Name

 Non-receptor tyrosine-protein kinase TNK1 

Protein Synonyms/Alias

 CD38 negative kinase 1 

Gene Name

 TNK1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
141	LWTLPSGKSVPVAVK	ubiquitination	[1, 2, 3]
260	LASPRTIKVADFGLV	ubiquitination	[2, 4]
423	SPDSTIWKGQNGRTF	ubiquitination	[2, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction. 

Sequence Annotation

 DOMAIN 116 377 Protein kinase.
 DOMAIN 381 444 SH3.
 NP_BIND 122 130 ATP (By similarity).
 ACT_SITE 245 245 Proton acceptor (By similarity).
 BINDING 148 148 ATP (By similarity).
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 255 255 Phosphoserine.
 MOD_RES 502 502 Phosphoserine.
 MOD_RES 514 514 Phosphothreonine.
 MOD_RES 519 519 Phosphoserine.
 MOD_RES 582 582 Phosphoserine.  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain; Transferase; Tyrosine-protein kinase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 666 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; NAS:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0030308; P:negative regulation of cell growth; IEA:Compara.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IEA:Compara.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR001452; SH3_domain.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom. 

Pfam

 PF07714; Pkinase_Tyr 

SMART

 SM00326; SH3
 SM00219; TyrKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR
 PS50002; SH3 

PRINTS

 PR00109; TYRKINASE.