CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001749
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate dehydrogenase 1, mitochondrial 
Protein Synonyms/Alias
 GDH 1 
Gene Name
 GLUD1 
Gene Synonyms/Alias
 GLUD 
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
84GASIVEDKLVEDLKTsuccinylation[1]
110RSILRIIKPCNHVLSsuccinylation[1]
162DVSVDEVKALASLMTsuccinylation[1]
363GTILGFPKAKIYEGSsuccinylation[1]
415PTTPEADKIFLERNIsuccinylation[1]
457SYGRLTFKYERDSNYmalonylation[1]
457SYGRLTFKYERDSNYsuccinylation[1]
503RISGASEKDIVHSGLmalonylation[1]
503RISGASEKDIVHSGLsuccinylation[1]
527QIMRTAMKYNLGLDLmalonylation[1]
527QIMRTAMKYNLGLDLsuccinylation[1]
545AYVNAIEKVFRVYNEsuccinylation[1]
Reference
 [1] Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase.
 Du J, Zhou Y, Su X, Yu JJ, Khan S, Jiang H, Kim J, Woo J, Kim JH, Choi BH, He B, Chen W, Zhang S, Cerione RA, Auwerx J, Hao Q, Lin H.
 Science. 2011 Nov 11;334(6057):806-9. [PMID: 22076378
Functional Description
 May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity). 
Sequence Annotation
 NP_BIND 141 143 NAD.
 ACT_SITE 183 183
 BINDING 147 147 Substrate.
 BINDING 171 171 Substrate.
 BINDING 176 176 NAD.
 BINDING 252 252 NAD.
 BINDING 266 266 GTP.
 BINDING 270 270 GTP.
 BINDING 319 319 GTP.
 BINDING 322 322 GTP.
 BINDING 438 438 Substrate.
 BINDING 444 444 NAD.
 BINDING 450 450 ADP.
 BINDING 516 516 ADP.
 MOD_RES 84 84 N6-acetyllysine; alternate.
 MOD_RES 84 84 N6-succinyllysine; alternate.
 MOD_RES 90 90 N6-acetyllysine.
 MOD_RES 110 110 N6-acetyllysine; alternate.
 MOD_RES 110 110 N6-succinyllysine; alternate.
 MOD_RES 135 135 Phosphotyrosine (By similarity).
 MOD_RES 162 162 N6-acetyllysine; alternate.
 MOD_RES 162 162 N6-succinyllysine; alternate.
 MOD_RES 172 172 ADP-ribosylcysteine (By similarity).
 MOD_RES 183 183 N6-acetyllysine.
 MOD_RES 191 191 N6-acetyllysine.
 MOD_RES 227 227 Phosphoserine (By similarity).
 MOD_RES 363 363 N6-acetyllysine; alternate.
 MOD_RES 363 363 N6-succinyllysine; alternate.
 MOD_RES 365 365 N6-acetyllysine.
 MOD_RES 386 386 N6-acetyllysine.
 MOD_RES 399 399 N6-acetyllysine.
 MOD_RES 415 415 N6-acetyllysine; alternate.
 MOD_RES 415 415 N6-succinyllysine; alternate.
 MOD_RES 450 450 Phosphoserine (By similarity).
 MOD_RES 457 457 N6-acetyllysine; alternate.
 MOD_RES 457 457 N6-malonyllysine; alternate.
 MOD_RES 457 457 N6-succinyllysine; alternate.
 MOD_RES 480 480 N6-acetyllysine.
 MOD_RES 503 503 N6-acetyllysine; alternate.
 MOD_RES 503 503 N6-malonyllysine; alternate.
 MOD_RES 503 503 N6-succinyllysine; alternate.
 MOD_RES 512 512 Phosphotyrosine (By similarity).
 MOD_RES 527 527 N6-acetyllysine; alternate.
 MOD_RES 527 527 N6-malonyllysine; alternate.
 MOD_RES 527 527 N6-succinyllysine; alternate.
 MOD_RES 545 545 N6-acetyllysine; alternate.
 MOD_RES 545 545 N6-succinyllysine; alternate.  
Keyword
 3D-structure; Acetylation; ADP-ribosylation; ATP-binding; Complete proteome; Direct protein sequencing; GTP-binding; Mitochondrion; NADP; Nucleotide-binding; Oxidoreductase; Phosphoprotein; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 558 AA 
Protein Sequence
MYRYLGEALL LSRAGPAALG SASADSAALL GWARGQPAAA PQPGLVPPAR RHYSEAAADR 60
EDDPNFFKMV EGFFDRGASI VEDKLVEDLK TRETEEQKRN RVRSILRIIK PCNHVLSLSF 120
PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG 180
GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGVD VPAPDMSTGE REMSWIADTY 240
ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG 300
DKTFVVQGFG NVGLHSMRYL HRFGAKCITV GESDGSIWNP DGIDPKELED FKLQHGTILG 360
FPKAKIYEGS ILEVDCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER 420
NIMVIPDLYL NAGGVTVSYF EWLNNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK 480
HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV 540
NAIEKVFRVY NEAGVTFT 558 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; ISS:AgBase.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004352; F:glutamate dehydrogenase (NAD+) activity; ISS:AgBase.
 GO:0004353; F:glutamate dehydrogenase [NAD(P)+] activity; ISS:AgBase.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0006538; P:glutamate catabolic process; ISS:AgBase.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara. 
Interpro
 IPR006095; Glu/Leu/Phe/Val_DH.
 IPR006096; Glu/Leu/Phe/Val_DH_C.
 IPR006097; Glu/Leu/Phe/Val_DH_dimer_dom.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00208; ELFV_dehydrog
 PF02812; ELFV_dehydrog_N 
SMART
 SM00839; ELFV_dehydrog 
PROSITE
 PS00074; GLFV_DEHYDROGENASE 
PRINTS
 PR00082; GLFDHDRGNASE.