CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017276
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Syntaxin-binding protein 5 
Protein Synonyms/Alias
 Lethal(2) giant larvae protein homolog 3; Tomosyn-1 
Gene Name
 Stxbp5 
Gene Synonyms/Alias
 Kiaa4253; Llgl3 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
766LSLPTDLKPDLDVKDsumoylation[1]
925ICSEKQAKVMSLPTQubiquitination[2]
Reference
 [1] Structural and functional analysis of tomosyn identifies domains important in exocytotic regulation.
 Williams AL, Bielopolski N, Meroz D, Lam AD, Passmore DR, Ben-Tal N, Ernst SA, Ashery U, Stuenkel EL.
 J Biol Chem. 2011 Apr 22;286(16):14542-53. [PMID: 21330375]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release (By similarity). Inhibits membrane fusion between transport vesicles and the plasma membrane. May modulate the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane. Competes with STXBP1 for STX1 binding. Inhibits translocation of GLUT4 from intracellular vesicles to the plasma membrane. 
Sequence Annotation
 REPEAT 62 95 WD 1.
 REPEAT 102 141 WD 2.
 REPEAT 146 182 WD 3.
 REPEAT 201 235 WD 4.
 REPEAT 241 273 WD 5.
 REPEAT 295 337 WD 6.
 REPEAT 345 379 WD 7.
 REPEAT 401 478 WD 8.
 REPEAT 506 620 WD 9.
 REPEAT 634 696 WD 10.
 REPEAT 795 852 WD 11.
 REPEAT 861 935 WD 12.
 REPEAT 940 984 WD 13.
 REPEAT 998 1021 WD 14.
 DOMAIN 1087 1147 v-SNARE coiled-coil homology.
 MOD_RES 724 724 Phosphoserine; by PKA (By similarity).
 MOD_RES 760 760 Phosphoserine (By similarity).
 MOD_RES 763 763 Phosphothreonine (By similarity).
 MOD_RES 783 783 Phosphoserine.
 MOD_RES 786 786 Phosphoserine.
 MOD_RES 1059 1059 Phosphoserine.  
Keyword
 Alternative splicing; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Exocytosis; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1152 AA 
Protein Sequence
MRKFNIRKVL DGLTAGSSSA SQQQQQQQHP PGNREPEIQE TLQSEHFQLC KTVRHGFPYQ 60
PSALAFDPVQ KILAVGTQTG ALRLFGRPGV ECYCQHDSGA AVIQLQFLIN EGALVSALAD 120
DTLHLWNLRQ KRPAVLHSLK FCRERVTFCH LPFQSKWLYV GTERGNIHIV NVESFTLSGY 180
VIMWNKAIEL SSKAHPGPVV HISDNPMDEG KLLIGFESGT VVLWDLKSKK ADYRYTYDEA 240
IHSVAWHHEG KQFICSHSDG TLTIWNVRSP AKPVQTITPH GKQLKDGKKP EPCKPILKVE 300
LKTTRSGEPF IILSGGLSYD TVGRRPCLTV MHGKSTAVLE MDYSIVDFLT LCETPYPNDF 360
QEPYAVVVLL EKDLVLIDLA QNGYPIFENP YPLSIHESPV TCCEYFADCP VDLIPALYSV 420
GARQKRQGYS KKEWPINGGN WGLGAQSYPE IIITGHADGS VKFWDASAIT LQVLYKLKTS 480
KVFEKSRNKD DRQNTDIVDE DPYAIQIISW CPESRMLCIA GVSAHVIIYR FSKQEVLTEV 540
IPMLEVRLLY EINDVDTPEG EQPPPLSTPV GSSNPQPIPP QSHPSTSSSS SDGLRDNVPC 600
LKVKNSPLKQ SPGYQTELVI QLVWVGGEPP QQITSLALNS SYGLVVFGNC NGIAMVDYLQ 660
KAVLLNLSTI ELYGSNDPYR REPRSPRKSR QPSGAGLCDI TEGTVVPEDR CKSPTSGSSS 720
PHNSDDEQKV NNFIEKVKTQ SRKFSKMVAN DLAKMSRKLS LPTDLKPDLD VKDNSFSRSR 780
SSSVTSIDKE SRETISALHF CETLTRKADS SPSPCLWVGT TVGTAFVITL NLPPGPEQRL 840
LQPVIVSPSG TILRLKGAIL RMAFLDATGC LMSPAYEPWK EHNVAEEKDE KEKLKKRRPV 900
SVSPSSSQEI SENQYAVICS EKQAKVMSLP TQSCAYKQNI TETSFVLRGD IVALSNSVCL 960
ACFCANGHIM TFSLPSLRPL LDVYYLPLTN MRIARTFCFA NNGQALYLVS PTEIQRLTYS 1020
QETCENLQEM LGELFTPVET PEAPNRGFFK GLFGGGAQSL DREELFGESS SGKASRSLAQ 1080
HIPGPGGIEG VKGAASGVVG ELARARLALD ERGQKLSDLE ERTAAMMSSA DSFSKHAHEM 1140
MLKYKDKKWY QF 1152 
Gene Ontology
 GO:0005892; C:acetylcholine-gated channel complex; IDA:MGI.
 GO:0030141; C:secretory granule; NAS:BHF-UCL.
 GO:0017075; F:syntaxin-1 binding; IDA:BHF-UCL.
 GO:0007409; P:axonogenesis; TAS:BHF-UCL.
 GO:0006887; P:exocytosis; IDA:MGI.
 GO:0045921; P:positive regulation of exocytosis; IDA:BHF-UCL.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR000664; Lethal2_giant.
 IPR013905; Lgl_C.
 IPR013577; LLGL2.
 IPR001388; Synaptobrevin.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08596; Lgl_C
 PF08366; LLGL
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS50892; V_SNARE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00962; LETHAL2GIANT.