UniProt Accession

 SC5D_HUMAN; O75845; O00119; Q9UK15 

Genbank Protein ID

 D85181; AF187981; AB016247; AB057650; BC012333 

Genbank Nucleotide ID

 BAA18970.1; AAF00544.1; BAA33729.1; BAB68218.1; AAH12333.1 

Protein Name

 Lathosterol oxidase 

Protein Synonyms/Alias

 C-5 sterol desaturase; Delta(7)-sterol 5-desaturase; Lathosterol 5-desaturase; Sterol-C5-desaturase 

Gene Name

 SC5D 

Gene Synonyms/Alias

 SC5DL 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
70	MKHPQFLKNQVRREI	ubiquitination	[1, 2, 3, 4]
255	DRIGGSFKNPSSFEG	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
263	NPSSFEGKGPLSYVK	ubiquitination	[1, 3, 4, 5, 7, 8]
289	GNGCKNEKLFNGEFT	ubiquitination	[3, 7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Catalyzes a dehydrogenation to introduce C5-6 double bond into lathosterol. 

Sequence Annotation

 MOTIF 138 143 Histidine box-1.
 MOTIF 151 155 Histidine box-2.
 MOTIF 228 233 Histidine box-3.
 MOD_RES 253 253 Phosphoserine.  

Keyword

 Complete proteome; Disease mutation; Endoplasmic reticulum; Iron; Lipid biosynthesis; Lipid metabolism; Membrane; NAD; NADP; Oxidoreductase; Phosphoprotein; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 299 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0000248; F:C-5 sterol desaturase activity; TAS:ProtInc.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0050046; F:lathosterol oxidase activity; IEA:EC.
 GO:0006695; P:cholesterol biosynthetic process; TAS:Reactome.
 GO:0033490; P:cholesterol biosynthetic process via lathosterol; IEA:Compara.
 GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro. 

Interpro

 IPR006694; Fatty_acid_hydroxylase. 

Pfam

 PF04116; FA_hydroxylase 

SMART

  

PROSITE

  

PRINTS