CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016350
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Homeodomain-interacting protein kinase 1 
Protein Synonyms/Alias
 Nuclear body-associated kinase 2 
Gene Name
 HIPK1 
Gene Synonyms/Alias
 KIAA0630; MYAK; NBAK2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
307ATALMKLKSLGLIHAubiquitination[1]
421YLLSAGTKTTRFFNRubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti- oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. 
Sequence Annotation
 DOMAIN 190 518 Protein kinase.
 NP_BIND 196 204 ATP (Probable).
 REGION 844 847 Nuclear localization signal 1 (NLS1) (By
 REGION 885 1093 Interaction with TP53.
 REGION 891 998 Required for localization to nuclear
 REGION 902 926 SUMO interaction motifs (SIM); required
 ACT_SITE 315 315 Proton acceptor (Probable).
 BINDING 219 219 ATP (Probable).
 MOD_RES 352 352 Phosphotyrosine (By similarity).
 MOD_RES 872 872 Phosphoserine.
 CROSSLNK 25 25 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1203 1203 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Kinase; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transcription; Transcription regulation; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1210 AA 
Protein Sequence
MASQLQVFSP PSVSSSAFCS AKKLKIEPSG WDVSGQSSND KYYTHSKTLP ATQGQANSSH 60
QVANFNIPAY DQGLLLPAPA VEHIVVTAAD SSGSAATSTF QSSQTLTHRS NVSLLEPYQK 120
CGLKRKSEEV DSNGSVQIIE EHPPLMLQNR TVVGAAATTT TVTTKSSSSS GEGDYQLVQH 180
EILCSMTNSY EVLEFLGRGT FGQVAKCWKR STKEIVAIKI LKNHPSYARQ GQIEVSILSR 240
LSSENADEYN FVRSYECFQH KNHTCLVFEM LEQNLYDFLK QNKFSPLPLK YIRPILQQVA 300
TALMKLKSLG LIHADLKPEN IMLVDPVRQP YRVKVIDFGS ASHVSKAVCS TYLQSRYYRA 360
PEIILGLPFC EAIDMWSLGC VIAELFLGWP LYPGASEYDQ IRYISQTQGL PAEYLLSAGT 420
KTTRFFNRDP NLGYPLWRLK TPEEHELETG IKSKEARKYI FNCLDDMAQV NMSTDLEGTD 480
MLAEKADRRE YIDLLKKMLT IDADKRITPL KTLNHQFVTM THLLDFPHSN HVKSCFQNME 540
ICKRRVHMYD TVSQIKSPFT THVAPNTSTN LTMSFSNQLN TVHNQASVLA SSSTAAAATL 600
SLANSDVSLL NYQSALYPSS AAPVPGVAQQ GVSLQPGTTQ ICTQTDPFQQ TFIVCPPAFQ 660
TGLQATTKHS GFPVRMDNAV PIVPQAPAAQ PLQIQSGVLT QGSCTPLMVA TLHPQVATIT 720
PQYAVPFTLS CAAGRPALVE QTAAVLQAWP GGTQQILLPS TWQQLPGVAL HNSVQPTAMI 780
PEAMGSGQQL ADWRNAHSHG NQYSTIMQQP SLLTNHVTLA TAQPLNVGVA HVVRQQQSSS 840
LPSKKNKQSA PVSSKSSLDV LPSQVYSLVG SSPLRTTSSY NSLVPVQDQH QPIIIPDTPS 900
PPVSVITIRS DTDEEEDNKY KPSSSGLKPR SNVISYVTVN DSPDSDSSLS SPYSTDTLSA 960
LRGNSGSVLE GPGRVVADGT GTRTIIVPPL KTQLGDCTVA TQASGLLSNK TKPVASVSGQ 1020
SSGCCITPTG YRAQRGGTSA AQPLNLSQVS AMGYCLLFGP CTVVTFWRTL LLAGC 1075 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:Compara.
 GO:0016605; C:PML body; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0009952; P:anterior/posterior pattern specification; IEA:Compara.
 GO:0006915; P:apoptotic process; IDA:UniProtKB.
 GO:0060216; P:definitive hemopoiesis; ISS:UniProtKB.
 GO:0048596; P:embryonic camera-type eye morphogenesis; IEA:Compara.
 GO:0060059; P:embryonic retina morphogenesis in camera-type eye; IEA:Compara.
 GO:0001654; P:eye development; ISS:UniProtKB.
 GO:0042771; P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; IEA:Compara.
 GO:0061072; P:iris morphogenesis; IEA:Compara.
 GO:0060235; P:lens induction in camera-type eye; IEA:Compara.
 GO:0030182; P:neuron differentiation; IEA:Compara.
 GO:0045766; P:positive regulation of angiogenesis; ISS:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0010803; P:regulation of tumor necrosis factor-mediated signaling pathway; IDA:UniProtKB.
 GO:0010842; P:retina layer formation; IEA:Compara.
 GO:0007224; P:smoothened signaling pathway; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS