UniProt Accession

 PSMD4_MOUSE; O35226; Q91V59; Q9JJM0; Q9JJM1; Q9JJM2; Q9JJM3 

Genbank Protein ID

 AF013099; AB029090; AB029142; AB029143; AB029144; AB029145; AB029146; AF175574; BC009005 

Genbank Nucleotide ID

 AAC53547.1; BAA97572.1; BAA97573.1; BAA97574.1; BAA97575.1; BAA97576.1; BAA97577.1; AAG09199.1; AAH09005.1 

Protein Name

 26S proteasome non-ATPase regulatory subunit 4 

Protein Synonyms/Alias

 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; Multiubiquitin chain-binding protein 

Gene Name

 Psmd4 

Gene Synonyms/Alias

 Mcb1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
40	VNIVCHSKTRSNPEN	ubiquitination	[1]
74	DTGRILSKLHTVQPK	ubiquitination	[1, 2]
122	SPVEDNEKDLVKLAK	acetylation	[3]
122	SPVEDNEKDLVKLAK	ubiquitination	[1]
126	DNEKDLVKLAKRLKK	ubiquitination	[1]
135	AKRLKKEKVNVDIIN	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Binds and presumably selects ubiquitin-conjugates for destruction. 

Sequence Annotation

 DOMAIN 5 188 VWFA.
 REPEAT 211 230 UIM 1.
 REPEAT 282 301 UIM 2.
 MOD_RES 250 250 Phosphothreonine.
 MOD_RES 253 253 Phosphothreonine.
 MOD_RES 256 256 Phosphoserine.
 MOD_RES 360 360 Phosphoserine (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Phosphoprotein; Proteasome; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 376 AA 

Protein Sequence

Gene Ontology

 GO:0022624; C:proteasome accessory complex; IDA:UniProtKB.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR027040; Proteasome_su_Rpn10.
 IPR003903; Ubiquitin-int_motif.
 IPR002035; VWF_A. 

Pfam

 PF02809; UIM 

SMART

 SM00726; UIM
 SM00327; VWA 

PROSITE

 PS50330; UIM
 PS50234; VWFA 

PRINTS