CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021890
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Thioredoxin reductase 1, cytoplasmic 
Protein Synonyms/Alias
 TR; Thioredoxin reductase TR1 
Gene Name
 Txnrd1 
Gene Synonyms/Alias
 Trxr1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
182NVGCIPKKLMHQAALacetylation[1]
182NVGCIPKKLMHQAALsuccinylation[1]
195ALLGQALKDSRNYGWubiquitination[2]
203DSRNYGWKVEDTVKHacetylation[3]
203DSRNYGWKVEDTVKHubiquitination[2]
209WKVEDTVKHDWEKMTacetylation[3]
209WKVEDTVKHDWEKMTubiquitination[2]
238RVALREKKVVYENAYubiquitination[2]
260RIVATNNKGKEKIYSubiquitination[2]
349FDQDMANKIGEHMEEubiquitination[2]
369IRQFVPTKIEQIEAGubiquitination[2]
422GLETVGVKINEKTGKubiquitination[2]
544AKIICNLKDDERVVGacetylation[3]
544AKIICNLKDDERVVGubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
  
Sequence Annotation
 NP_BIND 156 173 FAD (By similarity).
 ACT_SITE 586 586 Proton acceptor (By similarity).
 MOD_RES 245 245 Phosphotyrosine (By similarity).
 DISULFID 173 178 Redox-active (By similarity).
 CROSSLNK 611 612 Cysteinyl-selenocysteine (Cys-Sec) (By  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; Disulfide bond; FAD; Flavoprotein; NADP; Oxidoreductase; Phosphoprotein; Redox-active center; Reference proteome; Selenocysteine. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 613 AA 
Protein Sequence
MPVDDCWLYF PASRGRTFVQ TVWVAPTCPN CCWFPGFLPP VPRPPHVPRV LLRGPRGAVL 60
PASRPSKTLP SSSQTPCPTD PCICPPPSTP DSRQEKNTQS ELPNKKGQLQ KLPTMNGSKD 120
PPGSYDFDLI IIGGGSGGLA AAKEAAKFDK KVLVLDFVTP TPLGTRWGLG GTCVNVGCIP 180
KKLMHQAALL GQALKDSRNY GWKVEDTVKH DWEKMTESVQ SHIGSLNWGY RVALREKKVV 240
YENAYGRFIG PHRIVATNNK GKEKIYSAER FLIATGERPR YLGIPGDKEY CISSDDLFSL 300
PYCPGKTLVV GASYVALECA GFLAGIGLDV TVMVRSILLR GFDQDMANKI GEHMEEHGIK 360
FIRQFVPTKI EQIEAGTPGR LRVTAQSTNS EETIEGEFNT VLLAVGRDSC TRTIGLETVG 420
VKINEKTGKI PVTDEEQTNV PYIYAIGDIL EGKLELTPVA IQAGRLLAQR LYGGSNVKCD 480
YDNVPTTVFT PLEYGCCGLS EEKAVEKFGE ENIEVYHSFF WPLEWTVPSR DNNKCYAKII 540
CNLKDDERVV GFHVLGPNAG EVTQGFAAAL KCGLTKQQLD STIGIHPVCA EIFTTLSVTK 600
RSGGDILQSG CUG 613 
Gene Ontology
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0045340; F:mercury ion binding; IEA:Compara.
 GO:0016174; F:NAD(P)H oxidase activity; IEA:Compara.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0033797; F:selenate reductase activity; IEA:Compara.
 GO:0004791; F:thioredoxin-disulfide reductase activity; IDA:MGI.
 GO:0042537; P:benzene-containing compound metabolic process; IEA:Compara.
 GO:0008283; P:cell proliferation; IMP:MGI.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0071280; P:cellular response to copper ion; IEA:Compara.
 GO:0071455; P:cellular response to hyperoxia; IEA:Compara.
 GO:0006749; P:glutathione metabolic process; IEA:Compara.
 GO:0070276; P:halogen metabolic process; IEA:Compara.
 GO:0042744; P:hydrogen peroxide catabolic process; IEA:Compara.
 GO:0001707; P:mesoderm formation; IMP:MGI.
 GO:0042191; P:methylmercury metabolic process; IEA:Compara.
 GO:0070995; P:NADPH oxidation; IEA:Compara.
 GO:0001890; P:placenta development; IEA:Compara.
 GO:0010942; P:positive regulation of cell death; IEA:Compara.
 GO:0051262; P:protein tetramerization; IEA:Compara.
 GO:0048678; P:response to axon injury; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0010269; P:response to selenium ion; IEA:Compara.
 GO:0016259; P:selenocysteine metabolic process; IEA:Compara. 
Interpro
 IPR016156; FAD/NAD-linked_Rdtase_dimer.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR012999; Pyr_OxRdtase_I_AS.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom.
 IPR006338; Thioredoxin/glutathione_Rdtase. 
Pfam
 PF00070; Pyr_redox
 PF07992; Pyr_redox_2
 PF02852; Pyr_redox_dim 
SMART
  
PROSITE
 PS00076; PYRIDINE_REDOX_1 
PRINTS
 PR00368; FADPNR.