CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012309
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Frizzled-2 
Protein Synonyms/Alias
 Fz-2; hFz2; FzE2 
Gene Name
 FZD2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
206FHCPRVLKVPSYLSYubiquitination[1]
214VPSYLSYKFLGERDCubiquitination[2, 3]
446FRIRTIMKHDGTKTEmethylation[4]
500SWVSQHCKSLAIPCPubiquitination[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 
Functional Description
 Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. 
Sequence Annotation
 DOMAIN 34 153 FZ.
 MOTIF 543 548 Lys-Thr-X-X-X-Trp motif, mediates
 MOTIF 563 565 PDZ-binding.
 CARBOHYD 53 53 N-linked (GlcNAc...) (Potential).
 CARBOHYD 154 154 N-linked (GlcNAc...) (Potential).
 DISULFID 39 100 By similarity.
 DISULFID 47 93 By similarity.
 DISULFID 84 121 By similarity.
 DISULFID 110 150 By similarity.
 DISULFID 114 138 By similarity.  
Keyword
 Cell membrane; Complete proteome; Developmental protein; Disulfide bond; G-protein coupled receptor; Glycoprotein; Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix; Ubl conjugation; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 565 AA 
Protein Sequence
MRPRSALPRL LLPLLLLPAA GPAQFHGEKG ISIPDHGFCQ PISIPLCTDI AYNQTIMPNL 60
LGHTNQEDAG LEVHQFYPLV KVQCSPELRF FLCSMYAPVC TVLEQAIPPC RSICERARQG 120
CEALMNKFGF QWPERLRCEH FPRHGAEQIC VGQNHSEDGA PALLTTAPPP GLQPGAGGTP 180
GGPGGGGAPP RYATLEHPFH CPRVLKVPSY LSYKFLGERD CAAPCEPARP DGSMFFSQEE 240
TRFARLWILT WSVLCCASTF FTVTTYLVDM QRFRYPERPI IFLSGCYTMV SVAYIAGFVL 300
QERVVCNERF SEDGYRTVVQ GTKKEGCTIL FMMLYFFSMA SSIWWVILSL TWFLAAGMKW 360
GHEAIEANSQ YFHLAAWAVP AVKTITILAM GQIDGDLLSG VCFVGLNSLD PLRGFVLAPL 420
FVYLFIGTSF LLAGFVSLFR IRTIMKHDGT KTEKLERLMV RIGVFSVLYT VPATIVIACY 480
FYEQAFREHW ERSWVSQHCK SLAIPCPAHY TPRMSPDFTV YMIKYLMTLI VGITSGFWIW 540
SGKTLHSWRK FYTRLTNSRH GETTV 565 
Gene Ontology
 GO:0045177; C:apical part of cell; IBA:RefGenome.
 GO:0005737; C:cytoplasm; IDA:BHF-UCL.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0032589; C:neuron projection membrane; IBA:RefGenome.
 GO:0004930; F:G-protein coupled receptor activity; IEA:UniProtKB-KW.
 GO:0042813; F:Wnt-activated receptor activity; IBA:RefGenome.
 GO:0017147; F:Wnt-protein binding; IBA:RefGenome.
 GO:0007409; P:axonogenesis; IBA:RefGenome.
 GO:0007420; P:brain development; IBA:RefGenome.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IDA:UniProtKB.
 GO:0007267; P:cell-cell signaling; IEA:Compara.
 GO:0090103; P:cochlea morphogenesis; IEA:Compara.
 GO:0009790; P:embryo development; IBA:RefGenome.
 GO:0030855; P:epithelial cell differentiation; IBA:RefGenome.
 GO:0007199; P:G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger; IBA:RefGenome.
 GO:0008406; P:gonad development; IBA:RefGenome.
 GO:0060022; P:hard palate development; IEA:Compara.
 GO:0060119; P:inner ear receptor cell development; IEA:Compara.
 GO:0003149; P:membranous septum morphogenesis; IEA:Compara.
 GO:0003150; P:muscular septum morphogenesis; IEA:Compara.
 GO:0003151; P:outflow tract morphogenesis; IEA:Compara.
 GO:0090179; P:planar cell polarity pathway involved in neural tube closure; IEA:Compara.
 GO:0030825; P:positive regulation of cGMP metabolic process; IMP:BHF-UCL.
 GO:0051091; P:positive regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:BHF-UCL.
 GO:0007608; P:sensory perception of smell; IEA:Compara.
 GO:0001944; P:vasculature development; IBA:RefGenome.
 GO:0007223; P:Wnt receptor signaling pathway, calcium modulating pathway; IBA:RefGenome. 
Interpro
 IPR000539; Frizzled.
 IPR015526; Frizzled/SFRP.
 IPR020067; Frizzled_dom.
 IPR026550; FZD2.
 IPR017981; GPCR_2-like. 
Pfam
 PF01534; Frizzled
 PF01392; Fz 
SMART
 SM00063; FRI 
PROSITE
 PS50038; FZ
 PS50261; G_PROTEIN_RECEP_F2_4 
PRINTS
 PR00489; FRIZZLED.