UniProt Accession

 PI42A_MOUSE; O70172 

Genbank Protein ID

 AB009615; BC011097 

Genbank Nucleotide ID

 BAA25676.1; AAH11097.1 

Protein Name

 Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha 

Protein Synonyms/Alias

 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; Diphosphoinositide kinase 2-alpha; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; PI(5)P 4-kinase type II alpha; PIP4KII-alpha; PtdIns(5)P-4-kinase isoform 2-alpha 

Gene Name

 Pip4k2a 

Gene Synonyms/Alias

 Pip5k2a 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
91	MPSHFKFKEYCPMVF	ubiquitination	[1]
160	AEMHNILKKYHQYIV	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P (By similarity). May be involved in thrombopoiesis and the terminal maturation of megakaryocytes and regulation of their size. 

Sequence Annotation

 DOMAIN 33 405 PIPK.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 3 3 Phosphothreonine (By similarity).
 MOD_RES 14 14 Phosphoserine (By similarity).
 MOD_RES 89 89 N6-acetyllysine (By similarity).
 MOD_RES 145 145 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 405 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016309; F:1-phosphatidylinositol-5-phosphate 4-kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0035855; P:megakaryocyte development; IMP:UniProtKB.
 GO:0046854; P:phosphatidylinositol phosphorylation; IEA:GOC. 

Interpro

 IPR023610; PInositol-4-P-5-kinase.
 IPR027483; PInositol-4-P-5-kinase_C.
 IPR002498; PInositol-4-P-5-kinase_core.
 IPR027484; PInositol-4-P-5-kinase_N.
 IPR016034; PInositol-4P-5-kinase_core_sub. 

Pfam

 PF01504; PIP5K 

SMART

 SM00330; PIPKc 

PROSITE

 PS51455; PIPK 

PRINTS