CPLM-023140

Genbank Nucleotide ID

Serine/threonine-protein kinase TAO2

Protein Synonyms/Alias

Kinase from chicken homolog C; hKFC-C; Prostate-derived sterile 20-like kinase 1; PSK-1; PSK1; Prostate-derived STE20-like kinase 1; Thousand and one amino acid protein kinase 2

KIAA0881; MAP3K17; PSK; PSK1; UNQ2971/PRO7431

Homo sapiens (Human)

Position	Peptide	Type	References
153	NMIHRDVKAGNILLS	ubiquitination	[1, 2]
264	FVDSCLQKIPQDRPT	ubiquitination	[2]
314	LQYRKMKKILFQEAP	ubiquitination	[2]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation.

DOMAIN 28 281 Protein kinase.
NP_BIND 34 42 ATP (By similarity).
ACT_SITE 151 151 Proton acceptor (By similarity).
BINDING 57 57 ATP (By similarity).
MOD_RES 9 9 Phosphoserine.
MOD_RES 43 43 Phosphotyrosine (By similarity).
MOD_RES 181 181 Phosphoserine.
MOD_RES 486 486 Phosphoserine.
MOD_RES 777 777 Phosphoserine.
MOD_RES 825 825 Phosphoserine.
MOD_RES 827 827 Phosphoserine.

Alternative splicing; ATP-binding; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Kinase; Magnesium; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016023; C:cytoplasmic membrane-bounded vesicle; IDA:UniProtKB.
GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005730; C:nucleolus; IDA:HPA.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
GO:0030036; P:actin cytoskeleton organization; IDA:UniProtKB.
GO:0000186; P:activation of MAPKK activity; IDA:UniProtKB.
GO:0006915; P:apoptotic process; NAS:UniProtKB.
GO:0016477; P:cell migration; NAS:UniProtKB.
GO:0048041; P:focal adhesion assembly; IDA:UniProtKB.
GO:0031572; P:G2 DNA damage checkpoint; IMP:UniProtKB.
GO:0046330; P:positive regulation of JNK cascade; IDA:UniProtKB.
GO:0006612; P:protein targeting to membrane; NAS:UniProtKB.
GO:0001558; P:regulation of cell growth; NAS:UniProtKB.
GO:0008360; P:regulation of cell shape; IDA:UniProtKB.
GO:0051403; P:stress-activated MAPK cascade; IDA:UniProtKB.

IPR011009; Kinase-like_dom.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.

PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST