CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031553
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase DDX3X 
Protein Synonyms/Alias
 cDNA FLJ52848, highly similar to ATP-dependent RNA helicase DDX3X (EC 3.6.1.-) 
Gene Name
 DDX3X 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
162GDRSGFGKFERGGNSubiquitination[1, 2, 3, 4]
259KHAIPIIKEKRDLMAubiquitination[2, 4]
261AIPIIKEKRDLMACAubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 308 AA 
Protein Sequence
MRESRLISDL GGFAGVRSAA GRDWRPFWLG GLRPSRLAQP GFPSGGPGGT RCLRACEQGF 60
AGLDLNSSDN QSGGSTASKG RYIPPHLRNR EATKGFYDKD SSGWSSSKDK DAYSSFGSRS 120
DSRGKSSFFS DRGSGSRGRF DDRGRSDYDG IGSRGDRSGF GKFERGGNSR WCDKSDEDDW 180
SKPLPPSERL EQELFSGGNT GINFEKYDDI PVEATGNNCP PHIESFSDVE MGEIIMGNIE 240
LTRYTRPTPV QKHAIPIIKE KRDLMACAQT GKWKVWAPQT IPNLLGISTN ERVGSTDLRG 300
SQKIFIPI 308 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
  
SMART
  
PROSITE
 PS51195; Q_MOTIF 
PRINTS