CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019218
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DnaJ homolog subfamily A member 3, mitochondrial 
Protein Synonyms/Alias
 DnaJ protein Tid-1; hTid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog 
Gene Name
 DNAJA3 
Gene Synonyms/Alias
 HCA57; TID1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
134DDPKAKEKFSQLAEAacetylation[1]
152LSDEVKRKQYDAYGSubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma- mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. 
Sequence Annotation
 DOMAIN 93 158 J.
 REPEAT 236 243 CXXCXGXG motif.
 REPEAT 253 260 CXXCXGXG motif.
 REPEAT 275 282 CXXCXGXG motif.
 REPEAT 289 296 CXXCXGXG motif.
 ZN_FING 223 301 CR-type.
 METAL 236 236 Zinc 1 (By similarity).
 METAL 239 239 Zinc 1 (By similarity).
 METAL 253 253 Zinc 2 (By similarity).
 METAL 256 256 Zinc 2 (By similarity).
 METAL 275 275 Zinc 2 (By similarity).
 METAL 278 278 Zinc 2 (By similarity).
 METAL 289 289 Zinc 1 (By similarity).
 METAL 292 292 Zinc 1 (By similarity).
 MOD_RES 58 58 Omega-N-methylarginine; by CARM1.
 MOD_RES 238 238 Omega-N-methylarginine; by CARM1.
 MOD_RES 293 293 Omega-N-methylarginine; by CARM1.  
Keyword
 3D-structure; Alternative splicing; Apoptosis; Cell junction; Cell membrane; Chaperone; Complete proteome; Cytoplasm; Membrane; Metal-binding; Methylation; Mitochondrion; Phosphoprotein; Polymorphism; Postsynaptic cell membrane; Reference proteome; Repeat; Synapse; Transit peptide; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 480 AA 
Protein Sequence
MAARCSTRWL LVVVGTPRLP AISGRGARPP REGVVGAWLS RKLSVPAFAS SLTSCGPRAL 60
LTLRPGVSLT GTKHNPFICT ASFHTSAPLA KEDYYQILGV PRNASQKEIK KAYYQLAKKY 120
HPDTNKDDPK AKEKFSQLAE AYEVLSDEVK RKQYDAYGSA GFDPGASGSQ HSYWKGGPTV 180
DPEELFRKIF GEFSSSSFGD FQTVFDQPQE YFMELTFNQA AKGVNKEFTV NIMDTCERCN 240
GKGNEPGTKV QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIIISPCV VCRGAGQAKQ 300
KKRVMIPVPA GVEDGQTVRM PVGKREIFIT FRVQKSPVFR RDGADIHSDL FISIAQALLG 360
GTARAQGLYE TINVTIPPGT QTDQKIRMGG KGIPRINSYG YGDHYIHIKI RVPKRLTSRQ 420
QSLILSYAED ETDVEGTVNG VTLTSSGGST MDSSAGSKAR REAGEDEEGF LSKLKKMFTS 480 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0019897; C:extrinsic to plasma membrane; ISS:UniProtKB.
 GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
 GO:0042645; C:mitochondrial nucleoid; IDA:UniProtKB.
 GO:0031594; C:neuromuscular junction; ISS:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0045211; C:postsynaptic membrane; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0005133; F:interferon-gamma receptor binding; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005083; F:small GTPase regulator activity; IEA:Compara.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:UniProtKB.
 GO:0006924; P:activation-induced cell death of T cells; IEA:Compara.
 GO:0007569; P:cell aging; IEA:Compara.
 GO:0009790; P:embryo development; IEA:Compara.
 GO:0006264; P:mitochondrial DNA replication; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:UniProtKB.
 GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IDA:UniProtKB.
 GO:0060336; P:negative regulation of interferon-gamma-mediated signaling pathway; IDA:UniProtKB.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IDA:UniProtKB.
 GO:0006469; P:negative regulation of protein kinase activity; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0007528; P:neuromuscular junction development; IDA:UniProtKB.
 GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0042102; P:positive regulation of T cell proliferation; IEA:Compara.
 GO:0006457; P:protein folding; IDA:UniProtKB.
 GO:0050821; P:protein stabilization; IDA:UniProtKB.
 GO:0009408; P:response to heat; IEA:InterPro.
 GO:0034341; P:response to interferon-gamma; IDA:UniProtKB.
 GO:0071340; P:skeletal muscle acetylcholine-gated channel clustering; ISS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:Compara.
 GO:0033077; P:T cell differentiation in thymus; IEA:Compara. 
Interpro
 IPR012724; DnaJ.
 IPR002939; DnaJ_C.
 IPR001623; DnaJ_domain.
 IPR018253; DnaJ_domain_CS.
 IPR008971; HSP40/DnaJ_pept-bd.
 IPR001305; HSP_DnaJ_Cys-rich_dom. 
Pfam
 PF00226; DnaJ
 PF01556; DnaJ_C
 PF00684; DnaJ_CXXCXGXG 
SMART
 SM00271; DnaJ 
PROSITE
 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS51188; ZF_CR 
PRINTS
 PR00625; JDOMAIN.