CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005131
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA (uracil(54)-C(5))-methyltransferase 
Protein Synonyms/Alias
 tRNA(m5U54)-methyltransferase; RUMT 
Gene Name
 trmA 
Gene Synonyms/Alias
 b3965; JW3937 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
177ERLPVAGKEMIYRQVacetylation[1]
242VLATEIAKPSVAAAQacetylation[1]
311GLDSETEKMVQAYPRacetylation[1]
341ETLSQTHKVERLALFacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNAs. 
Sequence Annotation
 ACT_SITE 324 324 Nucleophile (By similarity).
 BINDING 190 190 S-adenosyl-L-methionine (By similarity).
 BINDING 218 218 S-adenosyl-L-methionine; via carbonyl
 BINDING 223 223 S-adenosyl-L-methionine (By similarity).
 BINDING 239 239 S-adenosyl-L-methionine (By similarity).
 BINDING 299 299 S-adenosyl-L-methionine (By similarity).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 366 AA 
Protein Sequence
MTPEHLPTEQ YEAQLAEKVV RLQSMMAPFS DLVPEVFRSP VSHYRMRAEF RIWHDGDDLY 60
HIIFDQQTKS RIRVDSFPAA SELINQLMTA MIAGVRNNPV LRHKLFQIDY LTTLSNQAVV 120
SLLYHKKLDD EWRQEAEALR DALRAQNLNV HLIGRATKTK IELDQDYIDE RLPVAGKEMI 180
YRQVENSFTQ PNAAMNIQML EWALDVTKGS KGDLLELYCG NGNFSLALAR NFDRVLATEI 240
AKPSVAAAQY NIAANHIDNV QIIRMAAEEF TQAMNGVREF NRLQGIDLKS YQCETIFVDP 300
PRSGLDSETE KMVQAYPRIL YISCNPETLC KNLETLSQTH KVERLALFDQ FPYTHHMECG 360
VLLTAK 366 
Gene Ontology
 GO:0019843; F:rRNA binding; IDA:EcoCyc.
 GO:0030697; F:S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity; IDA:EcoCyc.
 GO:0000049; F:tRNA binding; IDA:EcoCyc. 
Interpro
 IPR011869; TrmA_MeTrfase_proteobac.
 IPR010280; U5_MeTrfase. 
Pfam
 PF05958; tRNA_U5-meth_tr 
SMART
  
PROSITE
 PS01230; TRMA_1
 PS01231; TRMA_2 
PRINTS