CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007225
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ARF guanine-nucleotide exchange factor 2 
Protein Synonyms/Alias
  
Gene Name
 GEA2 
Gene Synonyms/Alias
 YEL022W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
264IGTNVESKEEGSQEDubiquitination[1]
298EEHIHSEKSTNGAEQubiquitination[1]
311EQLDIVQKTTRSNSRubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Activates the ARF proteins by exchanging bound GDP for free GTP. 
Sequence Annotation
 DOMAIN 570 714 SEC7.
 MOD_RES 46 46 Phosphoserine.
 MOD_RES 284 284 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1459 AA 
Protein Sequence
MSDREFVTVD PVTIIIKECI NLSTAMRKYS KFTSQSGVAA LLGGGSEIFS NQDDYLAHTF 60
NNLNTNKHND PFLSGFIQLR LMLNKLKNLD NIDSLTILQP FLLIVSTSSI SGYITSLALD 120
SLQKFFTLNI INESSQNYIG AHRATVNALT HCRFEGSQQL SDDSVLLKVV FLLRSIVDSP 180
YGDLLSNSII YDVLQTILSL ACNNRRSEVL RNAAQSTMIA VTVKIFSKLK TIEPVNVNQI 240
YINDESYTND VLKADTIGTN VESKEEGSQE DPIGMKVNNE EAISEDDGIE EEHIHSEKST 300
NGAEQLDIVQ KTTRSNSRIQ AYADDNYGLP VVRQYLNLLL SLIAPENELK HSYSTRIFGL 360
ELIQTALEIS GDRLQLYPRL FTLISDPIFK SILFIIQNTT KLSLLQATLQ LFTTLVVILG 420
NNLQLQIELT LTRIFSILLD DGTANNSSSE NKNKPSIIKE LLIEQISILW TRSPSFFTST 480
FINFDCNLDR ADVSINFLKA LTKLALPESA LTTTESVPPI CLEGLVSLVD DMFDHMKDID 540
REEFGRQKNE MEILKKRDRK TEFIECTNAF NEKPKKGIPM LIEKGFIASD SDKDIAEFLF 600
NNNNRMNKKT IGLLLCHPDK VSLLNEYIRL FDFSGLRVDE AIRILLTKFR LPGESQQIER 660
IIEAFSSAYC ENQDYDPSKI SDNAEDDIST VQPDADSVFI LSYSIIMLNT DLHNPQVKEH 720
MSFEDYSGNL KGCCNHKDFP FWYLDRIYCS IRDKEIVMPE EHHGNEKWFE DAWNNLISST 780
TVITEIKKDT QSVMDKLTPL ELLNFDRAIF KQVGPSIVST LFNIYVVASD DHISTRMITS 840
LDKCSYISAF FDFKDLFNDI LNSIAKGTTL INSSHDDELS TLAFEYGPMP LVQIKFEDTN 900
TEIPVSTDAV RFGRSFKGQL NTVVFFRIIR RNKDPKIFSK ELWLNIVNII LTLYEDLILS 960
PDIFPDLQKR LKLSNLPKPS PEISINKSKE SKGLLSTFAS YLKGDEEPTE EEIKSSKKAM 1020
ECIKSSNIAA SVFGNESNIT ADLIKTLLDS AKTEKNADNS RYFEAELLFI IELTIALFLF 1080
CKEEKELGKF ILQKVFQLSH TKGLTKRTVR RMLTYKILLI SLCADQTEYL SKLINDELLK 1140
KGDIFTQKFF ATNQGKEFLK RLFSLTESEF YRGFLLGNEN FWKFLRKVTA MKEQSESIFE 1200
YLNESIKTDS NILTNENFMW VLGLLDEISS MGAVGNHWEI EYKKLTESGH KIDKENPYKK 1260
SIELSLKSIQ LTSHLLEDNN DLRKNEIFAI IQALAHQCIN PCKQISEFAV VTLEQTLINK 1320
IEIPTNEMES VEELIEGGLL PLLNSSETQE DQKILISSIL TIISNVYLHY LKLGKTSNET 1380
FLKILSIFNK FVEDSDIEKK LQQLILDKKS IEKGNGSSSH GSAHEQTPES NDVEIEATAP 1440
IDDNTDDDNK PKLSDVEKD 1459 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0019898; C:extrinsic to membrane; IDA:SGD.
 GO:0000137; C:Golgi cis cisterna; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005086; F:ARF guanyl-nucleotide exchange factor activity; IDA:SGD.
 GO:0030036; P:actin cytoskeleton organization; IGI:SGD.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IMP:SGD.
 GO:0006891; P:intra-Golgi vesicle-mediated transport; IMP:SGD.
 GO:0016236; P:macroautophagy; IMP:SGD.
 GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; IGI:SGD.
 GO:0033363; P:secretory granule organization; IMP:SGD. 
Interpro
 IPR000904; Sec7.
 IPR023394; SEC7_alpha_orthog. 
Pfam
 PF01369; Sec7 
SMART
 SM00222; Sec7 
PROSITE
 PS50190; SEC7 
PRINTS