CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-026078
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA polymerase kappa 
Protein Synonyms/Alias
 Polymerase kappa isoform 1 
Gene Name
 POLK 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
371RTVTIKLKNVNFEVKubiquitination[1, 2]
378KNVNFEVKTRASTVSubiquitination[1]
433FPNEEDRKHQQRSIIubiquitination[1]
486DKKRSERKWSHQDTFubiquitination[1]
494WSHQDTFKCEAVNKQubiquitination[1]
513SQPFQVLKKKMNENLubiquitination[1]
514QPFQVLKKKMNENLEubiquitination[1]
551ISLEALNKHVDECLDubiquitination[1, 2]
593PASSLCEKQDYEAHPubiquitination[1, 2]
603YEAHPKIKEISSVDCubiquitination[1]
632SIDALSNKHSKEECSubiquitination[1]
644ECSSLPSKSFNIEHCubiquitination[1]
682QPYLCEVKTGQALVCubiquitination[1]
724IQELRKDKFNPVNQPubiquitination[1]
732FNPVNQPKESSRSTGubiquitination[1]
746GSSSGVQKAVTRTKRubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 780 AA 
Protein Sequence
MELEQSRNLS NTIVHIDMDA FYAAVEMRDN PELKDKPIAV GSMSMLSTSN YHARRFGVRA 60
AMPGFIAKRL CPQLIIVPPN FDKYRAVSKE VKEILADYDP NFMAMSLDEA YLNITKHLEE 120
RQNWPEDKRR YFIKMGSSVE NDNPGKEVNK LSEHERSISP LLFEESPSDV QPPGDPFQVN 180
FEEQNNPQIL QNSVVFGTSA QEVVKEIRFR IEQKTTLTAS AGIAPNTMLA KVCSDKNKPN 240
GQYQILPNRQ AVMDFIKDLP IRKVSGIGKV TEKMLKALGI ITCTELYQQR ALLSLLFSET 300
SWHYFLHISL GLGSTHLTRD GERKSMSVER TFSEINKAEE QYSLCQELCS ELAQDLQKER 360
LKGRTVTIKL KNVNFEVKTR ASTVSSVVST AEEIFAIAKE LLKTEIDADF PHPLRLRLMG 420
VRISSFPNEE DRKHQQRSII GFLQAGNQAL SATECTLEKT DKDKFVKPLE MSHKKSFFDK 480
KRSERKWSHQ DTFKCEAVNK QSFQTSQPFQ VLKKKMNENL EISENSDDCQ ILTCPVCFRA 540
QGCISLEALN KHVDECLDGP SISENFKMFS CSHVSATKVN KKENVPASSL CEKQDYEAHP 600
KIKEISSVDC IALVDTIDNS SKAESIDALS NKHSKEECSS LPSKSFNIEH CHQNSSSTVS 660
LENEDVGSFR QEYRQPYLCE VKTGQALVCP VCNVEQKTSD LTLFNVHVDV CLNKSFIQEL 720
RKDKFNPVNQ PKESSRSTGS SSGVQKAVTR TKRPGLMTKY STSKKIKPNN PKHTLDIFFK 780 
Gene Ontology
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:Compara. 
Interpro
 IPR017961; DNA_pol_Y-fam_little_finger.
 IPR001126; DNA_repair_prot_UmuC-like.
 IPR017963; DNA_repair_prot_UmuC-like_N.
 IPR022880; DNApol_IV.
 IPR024728; PolY_HhH_motif.
 IPR006642; Znf_Rad18_put. 
Pfam
 PF00817; IMS
 PF11799; IMS_C
 PF11798; IMS_HHH 
SMART
 SM00734; ZnF_Rad18 
PROSITE
 PS50173; UMUC 
PRINTS