CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032074
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial 
Protein Synonyms/Alias
 cDNA FLJ55034, highly similar to Dihydrolipoyllysine-residue succinyltransferase component of 2- oxoglutarate dehydrogenase complex, mitochondrial (EC 2.3.1.61) 
Gene Name
 DLST 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
117GKPVSAVKPTVAPPLubiquitination[1, 2]
131LAEPGAGKGLRSEHRubiquitination[1, 2]
221AVIDDTTKEVVYRDYacetylation[3]
221AVIDDTTKEVVYRDYubiquitination[4]
267TITELGEKARKNELAubiquitination[4, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Acyltransferase; Complete proteome; Lipoyl; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 367 AA 
Protein Sequence
MTWLQSKPQR LQNLSQREMS GGRKTSVQVP SPANGVIEAL LVPDGGKVEG GTPLFTLRKT 60
GAAPAKAKPA EAPAAAAPKA EPTAAAVPPP AAPIPTQMPP VPSPSQPPSG KPVSAVKPTV 120
APPLAEPGAG KGLRSEHREK MNRMRQRIAQ RLKEAQNTCA MLTTFNEIDM SNIQEMRARH 180
KEAFLKKHNL KLGFMSAFVK ASAFALQEQP VVNAVIDDTT KEVVYRDYID ISVAVATPRG 240
LVVPVIRNVE AMNFADIERT ITELGEKARK NELAIEDMDG GTFTISNGGV FGSLFGTPII 300
NPPQSAILGM HGIFDRPVAI GGKVEVRPMM YVALTYDHRL IDGREAVTFL RKIKAAVEDP 360
RVLLLDL 367 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0045252; C:oxoglutarate dehydrogenase complex; IEA:InterPro.
 GO:0004149; F:dihydrolipoyllysine-residue succinyltransferase activity; IEA:InterPro.
 GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro. 
Interpro
 IPR001078; 2-oxoacid_DH_actylTfrase.
 IPR000089; Biotin_lipoyl.
 IPR023213; CAT-like_dom.
 IPR011053; Single_hybrid_motif.
 IPR006255; SucB. 
Pfam
 PF00198; 2-oxoacid_dh
 PF00364; Biotin_lipoyl 
SMART
  
PROSITE
  
PRINTS