CPLM-004091

Genbank Nucleotide ID

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Protein Synonyms/Alias

Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1

Homo sapiens (Human)

Position	Peptide	Type	References
29	RAHESVVKSEDFSLP	ubiquitination	[1, 2, 3]
53	LPEVAHVKHLSASQK	acetylation	[4]
53	LPEVAHVKHLSASQK	ubiquitination	[1, 2, 3, 5]
60	KHLSASQKALKEKEK	acetylation	[4]
60	KHLSASQKALKEKEK	ubiquitination	[2]
67	KALKEKEKASWSSLS	ubiquitination	[2]
87	ELYRIKFKESFAEMN	acetylation	[6]
87	ELYRIKFKESFAEMN	ubiquitination	[2]
135	PLPQSFDKEWVAKQT	ubiquitination	[1, 3, 5]
140	FDKEWVAKQTKRMLD	ubiquitination	[5]
149	TKRMLDMKVNPIQGL	ubiquitination	[1, 2, 3, 5, 7, 8]
159	PIQGLASKWDYEKNE	ubiquitination	[1, 2, 3, 5]
164	ASKWDYEKNEWKK**	ubiquitination	[1, 3, 8]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[6] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
[7] A data set of human endogenous protein ubiquitination sites.
Shi Y, Chan DW, Jung SY, Malovannaya A, Wang Y, Qin J.
Mol Cell Proteomics. 2011 May;10(5):M110.002089. [PMID: 20972266]
[8] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

MOD_RES 53 53 N6-acetyllysine.
MOD_RES 60 60 N6-acetyllysine.

Acetylation; Complete proteome; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Polymorphism; Reference proteome; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
GO:0005634; C:nucleus; IDA:HPA.
GO:0004129; F:cytochrome-c oxidase activity; TAS:ProtInc.
GO:0022904; P:respiratory electron transport chain; TAS:Reactome.
GO:0007584; P:response to nutrient; IEA:Compara.
GO:0044281; P:small molecule metabolic process; TAS:Reactome.

IPR013288; Cyt_c_oxidase_su4.
IPR004203; Cyt_c_oxidase_su4_fam.

PR01873; CYTCOXIDASE4.