UniProt Accession

 RFA2_YEAST; P26754; D6W0N3; P38905 

Genbank Protein ID

 X59749; S64861; Z46259; Z71588; BK006947 

Genbank Nucleotide ID

 CAA42421.1; AAB27888.1; CAA96241.1; DAA10249.1 

Protein Name

 Replication factor A protein 2 

Protein Synonyms/Alias

 RF-A protein 2; DNA-binding protein BUF1; Replication protein A 36 kDa subunit 

Gene Name

 RFA2 

Gene Synonyms/Alias

 BUF1; YNL312W; N0368 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
100	TGQIEVRKWSEDAND	ubiquitination	[1]
141	VKVFGALKEFGGKKN	acetylation	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T- AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication. 

Sequence Annotation

 MOD_RES 27 27 Phosphoserine.
 MOD_RES 122 122 Phosphoserine.  

Keyword

 Complete proteome; Direct protein sequencing; DNA replication; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 273 AA 

Protein Sequence

Gene Ontology

 GO:0000781; C:chromosome, telomeric region; IMP:SGD.
 GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
 GO:0005662; C:DNA replication factor A complex; IDA:SGD.
 GO:0003690; F:double-stranded DNA binding; IDA:SGD.
 GO:0043565; F:sequence-specific DNA binding; IDA:SGD.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0006268; P:DNA unwinding involved in replication; IDA:SGD.
 GO:0000724; P:double-strand break repair via homologous recombination; IGI:SGD.
 GO:0045184; P:establishment of protein localization; IMP:SGD.
 GO:0030491; P:heteroduplex formation; IDA:SGD.
 GO:0006289; P:nucleotide-excision repair; IMP:SGD.
 GO:0016567; P:protein ubiquitination; IPI:SGD.
 GO:0007131; P:reciprocal meiotic recombination; IPI:SGD.
 GO:0000722; P:telomere maintenance via recombination; IPI:SGD.
 GO:0007004; P:telomere maintenance via telomerase; IMP:SGD. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase.
 IPR014646; RPA32.
 IPR014892; RPA_C.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF08784; RPA_C
 PF01336; tRNA_anti 

SMART

  

PROSITE

  

PRINTS