| Tag | Content |
|---|
| CPLM ID | CPLM-004755 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Mediator of RNA polymerase II transcription subunit 14 |
Protein Synonyms/Alias | Glucose repression regulatory protein 1; Mediator complex subunit 14 |
Gene Name | RGR1 |
Gene Synonyms/Alias | MED14; YLR071C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 25 | SNEMHALKNRSEQNG | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. |
Sequence Annotation | MOD_RES 2 2 N-acetylthreonine.
MOD_RES 7 7 Phosphoserine.
MOD_RES 1036 1036 Phosphothreonine. |
Keyword | Acetylation; Activator; Complete proteome; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1082 AA |
Protein Sequence | MTTTIGSPQM LANEERLSNE MHALKNRSEQ NGQEQQGPVK NTQLHGPSAT DPETTATQKE 60
SLEMVPKDTS AATMTSAPPP ALPHVEINQV SLALVIRNLT VFTMKELAQY MKTNVHTQAN 120
EPNSAKKIRF LQLIIFLRTQ FLKLYVLVKW TRTIKQNNFH VLIDLLNWFR TTNMNVNNCI 180
WALKSSLNSM TNAKLPNVDL VTALEVLSLG RPNLPTHNFK LSGVSNSMDM VDGMAKVPIG 240
LILQRLKDLN LTVSIKIALM NIPKPLNSYH IKNGRIYFTV PNEFEIQLST VNRQSPLFFV 300
DLKLLFNTEA EQTVSAVTEA TSTNGDSENN EENSSSNGNN LPLNKPRLEK LINEILLKSN 360
DPLLSLYNFL HKYVLTLQLY MVHREFLKLA NGGKFSKSNL IHNYDSKKST ITVRYWLNGK 420
MDSKGKITIG IQRTTESLIL KWDNQSASRA KNMPVIYNNI VSNIEGILDE IMFNHARIIR 480
SELLARDIFQ EDEENSDVLL FQLPTTCVSM APIQLKIDLL SGQFYFRNPT PLLSNYASKI 540
NRAEGPEELA RILQQLKLDK IIHVLTTMFE NTGWSCSRII KIDKPIRTQV NTGGESVVKK 600
EDNKYAIAGN STTNSDVSLL LQRDLFIRLP HWPLNWYLIL SIISSKTSCV VEKRIGKIVS 660
QRGKWNLKYL DNSNVMTVKL ESITYQKIMI LQRTILNRII NHMLIDSLNQ LEIRNKICSS 720
EMINEQKLPQ YIIQGSNTND NISIITLELE SFLEGSKALN SILESSMFLR IDYSNSQIRL 780
YAKFKRNTMM IQCQIDKLYI HFVQEEPLAF YLEESFTNLG IIVQYLTKFR QKLMQLVVLT 840
DVVERLHKNF ESENFKIIAL QPNEISFKYL SNNDEDDKDC TIKISTNDDS IKNLTVQLSP 900
SNPQHIIQPF LDNSKMDYHF IFSYLQFTSS LFKALKVILN ERGGKFHESG SQYSTMVNIG 960
LHNLNEYQIV YYNPQAGTKI TICIELKTVL HNGRDKIQFH IHFADVAHIT TKSPAYPMMH 1020
QVRNQVFMLD TKRLGTPESV KPANASHAIR LGNGVACDPS EIEPILMEIH NILKVDSNSS 1080
SS 1082 |
Gene Ontology | GO:0070847; C:core mediator complex; IDA:SGD. GO:0016592; C:mediator complex; IEA:InterPro. GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro. GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |