CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024311
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Brefeldin A-inhibited guanine nucleotide-exchange protein 2 
Protein Synonyms/Alias
 Brefeldin A-inhibited GEP 2; ADP-ribosylation factor guanine nucleotide-exchange factor 2 
Gene Name
 Arfgef2 
Gene Synonyms/Alias
 Arfgep2; Big2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
49EIKAELEKQRLGAAAacetylation[1]
309DVVTSAVKEAAEKHGubiquitination[2]
569QELSLRKKGLECLVSubiquitination[2]
872VEMEQMAKTAKALMEubiquitination[2]
1178LSMKFLEKGELANFRubiquitination[2]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extend on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways (By similarity). 
Sequence Annotation
 DOMAIN 661 792 SEC7.
 REGION 2 224 DCB; DCB:DCB domain and DCB:HUS domain
 REGION 515 535 HUS; DCB:HUS domain interaction (By
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 218 218 Phosphoserine.
 MOD_RES 227 227 Phosphoserine.
 MOD_RES 621 621 Phosphoserine.
 MOD_RES 623 623 Phosphothreonine.
 MOD_RES 1532 1532 Phosphoserine (By similarity).
 MOD_RES 1535 1535 Phosphoserine (By similarity).
 MOD_RES 1789 1789 Phosphoserine (By similarity).  
Keyword
 Cell junction; Cell projection; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endosome; Golgi apparatus; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Protein transport; Reference proteome; Synapse; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1792 AA 
Protein Sequence
MQESQTKSMF VSRALEKILA DKEVKRPQHS QLRRACQVAL DEIKAELEKQ RLGAAAPPKA 60
NFIEADKYFL PFELACQSKS PRVVSTSLDC LQKLIAYGHI TGNAPDSGAP GKRLIDRIVE 120
TICNCFQGPQ TDEGVQLQII KALLTAVTSP HIEIHEGTIL QTVRTCYNIY LASKNLINQT 180
TAKATLTQML NVIFTRMENQ VLQEARELEK PMQSKPQSPV IQATAGSPKF SRLKQSQAQS 240
KPTTPEKAEL PNGDHAQSGL GKVSLENGEA PRERGSPVSG RAEPSRGTDS GAQEVVKDIL 300
EDVVTSAVKE AAEKHGLPEP DRALGALECQ ECAVPPGVDE NSQTNGIADD RQSLSSADNL 360
EPDVQGHQVA ARFSHILQKD AFLVFRSLCK LSMKPLGEGP PDPKSHELRS KVVSLQLLLS 420
VLQNAGPVFR SHEMFVTAIK QYLCVALSKN GVSSVPDVFE LSLAIFLTLL SNFKMHLKMQ 480
IEVFFKEIFL NILETSTSSF EHRWMVIQTL TRICADAQCV VDIYVNYDCD LNAANIFERL 540
VNDLSKIAQG RSGHELGMTP LQELSLRKKG LECLVSILKC MVEWSKDLYV NPNHQATLGQ 600
ERLPDQEMGD GKGLDMARRC SVTSVESTVS SGTQTAIQDD PEQFEVIKQQ KEIIEHGIEL 660
FNKKPKRGIQ FLQEQGMLGA AVEDIAQFLH QEERLDSTQV GEFLGDSTRF NKEVMYAYVD 720
QLDFCEKEFV SALRTFLEGF RLPGEAQKID RLMEKFAARY IECNQGQTLF ASADTAYVLA 780
YSIIMLTTDL HSPQVKNKMT KEQYIKMNRG INDSKDLPEE YLSSIYDEIE GKKIAMKETK 840
EHTIATKSTK QSVASEKQRR LLYNVEMEQM AKTAKALMEA VSHAKAPFTS ATHLDHVRPM 900
FKLVWTPLLA AYSIGLQNCD DTEVASLCLE GIRCAVRIAC IFGMQLERDA YVQALARFSL 960
LTASSSITEM KQKNIDTIKT LITVAHTDGN YLGNSWHEIL KCISQLELAQ LIGTGVKTRY 1020
LSGSGREREG SLKGHSLAGE EFMGLGLGNL VSGGVDKRQM ASFQESVGET SSQSVVVAVD 1080
RIFTGSTRLD GNAIVDFVRW LCAVSMDELA SPHHPRMFSL QKIVEISYYN MNRIRLQWSR 1140
IWHVIGDHFN KVGCNPNEDV AIFAVDSLRQ LSMKFLEKGE LANFRFQKDF LRPFEHIMKK 1200
NRSPTIRDMV IRCIAQMVSS QAANIRSGWK NIFAVFHQAA SDHDGNIVEL AFQTTGHIVS 1260
TIFQHHFPAA IDSFQDAVKC LSEFACNAAF PDTSMEAIRL IRFCGKYVSE RPRVLQEYTS 1320
DDMNVAPGDR VWVRGWFPIL FELSCIINRC KLDVRTRGLT VMFEIMKSYG HTFAKHWWQD 1380
LFRIVFRIFD NMKLPEQQSE KSEWMTTTCN HALYAICDVF TQFYEALHEV LLSDVFAQLQ 1440
WCVKQDNEQL ARSGTNCLEN LVISNGEKFS PAVWDETCNC MLDIFKTTIP HVLLTWRPAG 1500
MEEEVSDRHL DVDLDRQSLS SIDRNASERG QSQLSNPTDD SWKGAPYAHQ KLLASLLIKC 1560
VVQLELIQTI DNIVFYPATS KKEDAEHMVA AQQDTLDAEI HIETENQGMY KFMSSQHLFK 1620
LLDCLQESHS FSKAFNSNYE QRTVLWRAGF KGKSKPNLLK QETSSLACCL RILFRMYVDE 1680
NRRDSWDEIQ QRLLRVCSEA LAYFITVNSE SHREAWTSLL LLLLTKTLKI SDEKFKAHAS 1740
MYYPYLCEIM QFDLIPELRA VLRKFFLRIG LVYKIWIPEE PSQVPAALSS TW 1792 
Gene Ontology
 GO:0032279; C:asymmetric synapse; ISS:UniProtKB.
 GO:0005879; C:axonemal microtubule; ISS:UniProtKB.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0016023; C:cytoplasmic membrane-bounded vesicle; IEA:UniProtKB-SubCell.
 GO:0031410; C:cytoplasmic vesicle; ISS:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0043197; C:dendritic spine; ISS:UniProtKB.
 GO:0000139; C:Golgi membrane; ISS:UniProtKB.
 GO:0005815; C:microtubule organizing center; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; ISS:UniProtKB.
 GO:0032280; C:symmetric synapse; ISS:UniProtKB.
 GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
 GO:0005086; F:ARF guanyl-nucleotide exchange factor activity; ISS:UniProtKB.
 GO:0050811; F:GABA receptor binding; ISS:UniProtKB.
 GO:0034237; F:protein kinase A regulatory subunit binding; ISS:UniProtKB.
 GO:0010256; P:endomembrane system organization; ISS:UniProtKB.
 GO:0007032; P:endosome organization; ISS:UniProtKB.
 GO:0006887; P:exocytosis; IEA:Compara.
 GO:0006893; P:Golgi to plasma membrane transport; ISS:UniProtKB.
 GO:0035556; P:intracellular signal transduction; IEA:Compara.
 GO:0032760; P:positive regulation of tumor necrosis factor production; ISS:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0001881; P:receptor recycling; ISS:UniProtKB.
 GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR015403; DUF1981_SEC7_assoc.
 IPR000904; Sec7.
 IPR023394; SEC7_alpha_orthog. 
Pfam
 PF09324; DUF1981
 PF01369; Sec7 
SMART
 SM00222; Sec7 
PROSITE
 PS50190; SEC7 
PRINTS