| Tag | Content |
|---|
| CPLM ID | CPLM-024224 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Adenylosuccinate synthetase |
Protein Synonyms/Alias | AMPSase; AdSS; IMP--aspartate ligase |
Gene Name | purA |
Gene Synonyms/Alias | MSMEG_0759; MSMEI_0743 |
Created Date | July 27, 2013 |
Organism | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) |
NCBI Taxa ID | 246196 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 292 | EHGAYLAKTGGEVGV | pupylation | [1] |
|
Reference | [1] Expansion of the mycobacterial "PUPylome". Watrous J, Burns K, Liu WT, Patel A, Hook V, Bafna V, Barry CE 3rd, Bark S, Dorrestein PC. Mol Biosyst. 2010 Feb;6(2):376-85. [ PMID: 20094657] |
Functional Description | Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity). |
Sequence Annotation | NP_BIND 12 18 GTP (By similarity).
NP_BIND 40 42 GTP (By similarity).
NP_BIND 331 333 GTP (By similarity).
NP_BIND 413 415 GTP (By similarity).
REGION 13 16 IMP binding (By similarity).
REGION 38 41 IMP binding (By similarity).
REGION 299 305 Substrate binding (By similarity).
ACT_SITE 13 13 Proton acceptor (By similarity).
ACT_SITE 41 41 Proton donor (By similarity).
METAL 13 13 Magnesium (By similarity).
METAL 40 40 Magnesium; via carbonyl oxygen (By
BINDING 129 129 IMP (By similarity).
BINDING 143 143 IMP; shared with dimeric partner (By
BINDING 224 224 IMP (By similarity).
BINDING 239 239 IMP (By similarity).
BINDING 303 303 IMP (By similarity).
BINDING 305 305 GTP (By similarity).
CROSSLNK 292 292 Isoglutamyl lysine isopeptide (Lys-Gln) |
Keyword | Complete proteome; Cytoplasm; GTP-binding; Isopeptide bond; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome; Ubl conjugation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 431 AA |
Protein Sequence | MPAIVLIGAQ WGDEGKGKAT DLLGGRVQWV VRYQGGNNAG HTVVLPTGEN FALHLIPSGI 60
LTPGVTNVIG NGVVVDPGVL LTELKGLEDR GVDTSNLLIS ADAHLLMPYH VAIDKVVERW 120
AGSKKIGTTG RGIGPCYQDK IARIGIRVAD VLDEQVLAEK IEAALEFKNQ VLVKIYNRKA 180
LEPAEVLENL LEQAEGFKHR IADARLLLNQ ALENDEAVLL EGSQGTLLDV DHGTYPFVTS 240
SNPTAGGAAV GSGIGPTRIT TVLGILKAYT TRVGSGPFPT ELFDEHGAYL AKTGGEVGVT 300
TGRARRCGWF DAVIARYATR VNGITDYFLT KLDVLSSLET VPVCVGYTVD GKRVDEMPMT 360
QSDIARAEPV YEELPGWWED ISGAREFEDL PAKARDYVLR LEELAGAYVS CIGVGPGRDQ 420
TIVRRDVLAA R 431 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0004019; F:adenylosuccinate synthase activity; IEA:HAMAP. GO:0005525; F:GTP binding; IEA:HAMAP. GO:0000287; F:magnesium ion binding; IEA:HAMAP. GO:0044208; P:'de novo' AMP biosynthetic process; IEA:UniProtKB-UniPathway. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |