UniProt Accession

 PKHA8_HUMAN; Q96JA3; B4DH00; Q7Z5V8; Q9BU78; Q9H274; Q9H8Z7 

Genbank Protein ID

 AF380162; AK023180; AK294857; CH471073; BC002838; BC053990; AF308300 

Genbank Nucleotide ID

 AAK55424.1; BAB14449.1; BAG57961.1; EAW93930.1; AAH02838.1; AAH53990.1; AAG48267.1 

Protein Name

 Pleckstrin homology domain-containing family A member 8 

Protein Synonyms/Alias

 PH domain-containing family A member 8; Phosphatidylinositol-four-phosphate adapter protein 2; FAPP-2; Phosphoinositol 4-phosphate adapter protein 2; hFAPP2; Serologically defined breast cancer antigen NY-BR-86 

Gene Name

 PLEKHA8 

Gene Synonyms/Alias

 FAPP2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
196	SPQLAMLKSSKMKHP	acetylation	[1]
416	KRGLKFLKGFLTEVK	ubiquitination	[2]

Reference

 [1] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans- Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation. 

Sequence Annotation

 DOMAIN 1 93 PH.
 REGION 310 519 Glycolipid transfer protein homology
 MOD_RES 139 139 Phosphothreonine (By similarity).
 MOD_RES 145 145 Phosphoserine (By similarity).
 MOD_RES 153 153 Phosphothreonine (By similarity).  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Golgi apparatus; Lipid transport; Lipid-binding; Membrane; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 519 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0097001; F:ceramide binding; IDA:UniProtKB.
 GO:0051861; F:glycolipid binding; IDA:UniProtKB.
 GO:0017089; F:glycolipid transporter activity; IDA:UniProtKB.
 GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:UniProtKB.
 GO:0035621; P:ER to Golgi ceramide transport; IDA:UniProtKB.
 GO:0046836; P:glycolipid transport; IEA:InterPro.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR014830; Glycolipid_transfer_prot_dom.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 

Pfam

 PF08718; GLTP
 PF00169; PH 

SMART

 SM00233; PH 

PROSITE

 PS50003; PH_DOMAIN 

PRINTS