CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000997
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General vesicular transport factor p115 
Protein Synonyms/Alias
 Protein USO1 homolog; Transcytosis-associated protein; TAP; Vesicle-docking protein 
Gene Name
 USO1 
Gene Synonyms/Alias
 VDP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
202RSNGAIQKIVAFENAacetylation[1]
202RSNGAIQKIVAFENAubiquitination[2, 3, 4]
580GKENFIEKLGFISKHubiquitination[2, 4]
705KDQYNLLKIQLGKDNubiquitination[2, 4]
919LLADQDQKILSLKNKubiquitination[2, 4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity). 
Sequence Annotation
 REPEAT 20 60 ARM 1.
 REPEAT 61 121 ARM 2.
 REPEAT 123 163 ARM 3.
 REPEAT 166 207 ARM 4.
 REPEAT 208 253 ARM 5.
 REPEAT 255 310 ARM 6.
 REPEAT 311 354 ARM 7.
 REPEAT 363 408 ARM 8.
 REPEAT 420 459 ARM 9.
 REPEAT 473 513 ARM 10.
 REPEAT 518 571 ARM 11.
 REPEAT 573 630 ARM 12.
 REGION 1 637 Globular head.
 MOD_RES 50 50 Phosphoserine.
 MOD_RES 202 202 N6-acetyllysine.
 MOD_RES 942 942 Phosphoserine.
 MOD_RES 952 952 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 962 AA 
Protein Sequence
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS KKYRLEVGIQ 60
AMEHLIHVLQ TDRSDSEIIG YALDTLYNII SNEEEEEVEE NSTRQSEDLG SQFTEIFIKQ 120
QENVTLLLSL LEEFDFHVRW PGVKLLTSLL KQLGPQVQQI ILVSPMGVSR LMDLLADSRE 180
VIRNDGVLLL QALTRSNGAI QKIVAFENAF ERLLDIISEE GNSDGGIVVE DCLILLQNLL 240
KNNNSNQNFF KEGSYIQRMK PWFEVGDENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT 300
SSCQKAMFQC GLLQQLCTIL MATGVPADIL TETINTVSEV IRGCQVNQDY FASVNAPSNP 360
PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNQKGQ GEIVSTLLPS TIDATGNSVS 420
AGQLLCGGLF STDSLSNWCA AVALAHALQE NATQKEQLLR VQLATSIGNP PVSLLQQCTN 480
ILSQGSKIQT RVGLLMLLCT WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL 540
CALLLGISIY FNDNSLESYM KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN 600
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN IVTHYKNMIR 660
EQDLQLEELR QQVSTLKCQN EQLQTAVTQQ VSQIQQHKDQ YNLLKIQLGK DNQHQGSYSE 720
GAQMNGIQPE EIGRLREEIE ELKRNQELLQ SQLTEKDSMI ENMKSSQTSG TNEQSSAIVS 780
ARDSEQVAEL KQELATLKSQ LNSQSVEITK LQTEKQELLQ KTEAFAKSVE VQGETETIIA 840
TKTTDVEGRL SALLQETKEL KNEIKALSEE RTAIKEQLDS SNSTIAILQT EKDKLELEIT 900
DSKKEQDDLL VLLADQDQKI LSLKNKLKDL GHPVEEEDEL ESGDQEDEDD ESEDPGKDLD 960
HI 962 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:ProtInc.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0048280; P:vesicle fusion with Golgi apparatus; IEA:InterPro. 
Interpro
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR006955; Uso1_p115_C.
 IPR024095; Vesicle_P115-like.
 IPR006953; Vesicle_Uso1_P115_head. 
Pfam
 PF04871; Uso1_p115_C
 PF04869; Uso1_p115_head 
SMART
  
PROSITE
 PS50176; ARM_REPEAT 
PRINTS