CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008183
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA replication licensing factor MCM4 
Protein Synonyms/Alias
 CDC21 homolog; P1-CDC21 
Gene Name
 Mcm4 
Gene Synonyms/Alias
 Cdc21; Mcmd4 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
818KGKTPALKYQQLFEDacetylation[1]
857DFLTVTGKTVRLL**acetylation[1, 2, 3]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). 
Sequence Annotation
 DOMAIN 457 666 MCM.
 NP_BIND 509 516 ATP (Potential).
 MOTIF 641 644 Arginine finger.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 31 31 Phosphoserine (By similarity).
 MOD_RES 32 32 Phosphoserine (By similarity).
 MOD_RES 119 119 Phosphoserine (By similarity).
 MOD_RES 130 130 Phosphoserine (By similarity).
 MOD_RES 141 141 Phosphoserine (By similarity).
 MOD_RES 144 144 Phosphoserine (By similarity).
 MOD_RES 219 219 N6-acetyllysine (By similarity).
 MOD_RES 449 449 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; ATP-binding; Cell cycle; Complete proteome; DNA replication; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 862 AA 
Protein Sequence
MSSPASTPSR RSSRRGRVTP TQSLRSEESR SSPNRRRRGE DSSTGELLPM PTSPGADLQS 60
PPAQNALFSS PPQMHSLAIP LDFDVSSPLT YGTPSSRVEG TPRSGVRGTP VRQRPDLGSA 120
RKGLQVDLQS DGAAAEDIVP SEQSLGQKLV IWGTDVNVAT CKENFQRFLQ CFTDPLAKEE 180
ENVGIDITQP LYMQQLGEIN ITGEPFLNVN CEHIKSFSKN LYRQLISYPQ EVIPTFDMAV 240
NEIFFDRYPD SILEHQIQVR PFNALKTKSM RNLNPEDIDQ LITISGMVIR TSQLIPEMQE 300
AFFQCQVCAH TTRVEIDRGR IAEPCSCVHC HTTHSMALIH NRSFFSDKQM IKLQESPEDM 360
PAGQTPHTIV LFAHNDLVDK VQPGDRVNVT GIYRAVPIRV NPRVSNVKSV YKTHIDVIHY 420
RKTDAKRLHG LDEEAEQKLF SEKRVKLLKE LSRKPDIYER LASALAPSIY EHEDIKKGIL 480
LQLFGGTRKD FSHTGRGKFR AEINILLCGD PGTSKSQLLQ YVYNLVPRGQ YTSGKGSSAV 540
GLTAYVMKDP ETRQLVLQTG ALVLSDNGIC CIDEFDKMNE STRSVLHEVM EQQTLSIAKA 600
GIICQLNART SVLAAANPIE SQWNPKKTTI ENIQLPHTLL SRFDLIFLML DPQDEAYDRR 660
LAHHLVSLYY QSEEQVEEEF LDMAVLKDYI AYAHSTIMPR LSEEASQALI EAYVNMRKIG 720
SSRGMVSAYP RQLESLIRLA EAHAKVRFSN KVEAIDVEEA KRLHREALKQ SATDPRTGIV 780
DISILTTGMS ATSRKRKEEL AEALRKLILS KGKTPALKYQ QLFEDIRGQS DTAITKDMFE 840
EALRALADDD FLTVTGKTVR LL 862 
Gene Ontology
 GO:0042555; C:MCM complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:Compara.
 GO:0003697; F:single-stranded DNA binding; IPI:MGI.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006270; P:DNA replication initiation; IEA:InterPro.
 GO:0006268; P:DNA unwinding involved in replication; IPI:MGI. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR008047; MCM_4.
 IPR018525; MCM_CS.
 IPR001208; MCM_DNA-dep_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase.
 IPR004039; Rubredoxin-type_fold. 
Pfam
 PF00493; MCM 
SMART
 SM00382; AAA
 SM00350; MCM 
PROSITE
 PS00847; MCM_1
 PS50051; MCM_2 
PRINTS
 PR01657; MCMFAMILY.
 PR01660; MCMPROTEIN4.