CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012352
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras GTPase-activating protein 3 
Protein Synonyms/Alias
 GAP1(IP4BP); Ins P4-binding protein 
Gene Name
 RASA3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
55FRTKIVEKSLCPFYGubiquitination[1]
104IQKEDLQKYHNRDTWubiquitination[1]
146DTGVVCHKLATRIVEubiquitination[1]
223FEEEDVDKLEIRVDLacetylation[2]
237LWNASNLKFGDEFLGubiquitination[1]
251GELRIPLKVLRQSSSubiquitination[1]
309PLRDLLLKSADVEPVubiquitination[1]
378RGNSLASKCIDETMKubiquitination[1]
641KLEEESFKMKNMFQVubiquitination[1, 3, 4]
643EEESFKMKNMFQVIQubiquitination[4]
746LYMSKLEKMQEACGSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Inhibitory regulator of the Ras-cyclic AMP pathway. Binds inositol tetrakisphosphate (IP4) with high affinity. Might be a specific IP4 receptor. 
Sequence Annotation
 DOMAIN 1 96 C2 1.
 DOMAIN 132 247 C2 2.
 DOMAIN 330 524 Ras-GAP.
 DOMAIN 576 677 PH.
 ZN_FING 679 715 Btk-type.
 MOD_RES 66 66 Phosphotyrosine (By similarity).
 MOD_RES 110 110 Phosphothreonine.
 MOD_RES 809 809 Phosphoserine.  
Keyword
 Cell membrane; Complete proteome; GTPase activation; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 834 AA 
Protein Sequence
MAVEDEGLRV FQSVKIKIGE AKNLPSYPGP SKMRDCYCTV NLDQEEVFRT KIVEKSLCPF 60
YGEDFYCEIP RSFRHLSFYI FDRDVFRRDS IIGKVAIQKE DLQKYHNRDT WFQLQHVDAD 120
SEVQGKVHLE LRLSEVITDT GVVCHKLATR IVECQGLPIV NGQCDPYATV TLAGPFRSEA 180
KKTKVKRKTN NPQFDEVFYF EVTRPCSYSK KSHFDFEEED VDKLEIRVDL WNASNLKFGD 240
EFLGELRIPL KVLRQSSSYE AWYFLQPRDN GSKSLKPDDL GSLRLNVVYT EDHVFSSDYY 300
SPLRDLLLKS ADVEPVSASA AHILGEVCRE KQEAAVPLVR LFLHYGRVVP FISAIASAEV 360
KRTQDPNTIF RGNSLASKCI DETMKLAGMH YLHVTLKPAI EEICQSHKPC EIDPVKLKDG 420
ENLENNMENL RQYVDRVFHA ITESGVSCPT VMCDIFFSLR EAAAKRFQDD PDVRYTAVSS 480
FIFLRFFAPA ILSPNLFQLT PHHTDPQTSR TLTLISKTVQ TLGSLSKSKS ASFKESYMAT 540
FYEFFNEQKY ADAVKNFLDL ISSSGRRDPK SVEQPIVLKE GFMIKRAQGR KRFGMKNFKK 600
RWFRLTNHEF TYHKSKGDQP LYSIPIENIL AVEKLEEESF KMKNMFQVIQ PERALYIQAN 660
NCVEAKDWID ILTKVSQCNQ KRLTVYHPSA YLSGHWLCCR APSDSAPGCS PCTGGLPANI 720
QLDIDGDRET ERIYSLFNLY MSKLEKMQEA CGSKSVYDGP EQEEYSTFVI DDPQETYKTL 780
KQVIAGVGAL EQEHAQYKRD KFKKTKYGSQ EHPIGDKSFQ NYIRQQSETS THSI 834 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0031235; C:intrinsic to internal side of plasma membrane; IDA:BHF-UCL.
 GO:0015278; F:calcium-release channel activity; IDA:BHF-UCL.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005099; F:Ras GTPase activator activity; IBA:RefGenome.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0046580; P:negative regulation of Ras protein signal transduction; IBA:RefGenome.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR001936; RasGAP.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot.
 IPR001562; Znf_Btk_motif. 
Pfam
 PF00779; BTK
 PF00168; C2
 PF00169; PH
 PF00616; RasGAP 
SMART
 SM00107; BTK
 SM00239; C2
 SM00233; PH
 SM00323; RasGAP 
PROSITE
 PS50004; C2
 PS50003; PH_DOMAIN
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2
 PS51113; ZF_BTK 
PRINTS
 PR00402; TECBTKDOMAIN.