CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029446
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein arginine N-methyltransferase 5 
Protein Synonyms/Alias
 Protein arginine methyltransferase 5, isoform CRA_c 
Gene Name
 PRMT5 
Gene Synonyms/Alias
 hCG_41976 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43EFIQEPAKNRPGPQTubiquitination[1, 2, 3, 4]
68WNTLIVGKLSPWIRPubiquitination[1, 2, 3, 4]
78PWIRPDSKVEKIRRNubiquitination[2, 4, 5]
81RPDSKVEKIRRNSEAubiquitination[4]
162VFEKDPIKYSQYQQAubiquitination[1, 3]
216KQADRRIKLYAVEKNubiquitination[1, 2]
308LAPISSSKLYNEVRAubiquitination[1, 3]
319EVRACREKDRDPEAQubiquitination[1, 2, 3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Methyltransferase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 466 AA 
Protein Sequence
MRGPNSGTEK GRLVIPEKQG FDFLCMPVFH PRFKREFIQE PAKNRPGPQT RSDLLLSGRD 60
WNTLIVGKLS PWIRPDSKVE KIRRNSEALE VQFIITGTNH HSEKEFCSYL QYLEYLSQNR 120
PPPNAYELFA KGYEDYLQSP LQPLMDNLES QTYEVFEKDP IKYSQYQQAI YKCLLDRVPE 180
EEKDTNVQVL MVLGAGRGPL VNASLRAAKQ ADRRIKLYAV EKNPNAVVTL ENWQFEEWGS 240
QVTVVSSDMR EWVAPEKADI IVSELLGSFA DNELSPECLD GAQHFLKDDG VSIPGEYTSF 300
LAPISSSKLY NEVRACREKD RDPEAQFEMP YVVRLHNFHQ LSAPQPCFTF SHPNRDPMID 360
NNRYCTLEFP VEVNTVLHGF AGYFETVLYQ DITLSIRPET HSPGMFSWFP ILFPIKQPIT 420
VREGQTICVR FWRCSNSKKV WYEWAVTAPV CSAIHNPTGR SYTIGL 466 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0006479; P:protein methylation; IEA:InterPro. 
Interpro
 IPR025799; Arg_MeTrfase.
 IPR007857; Arg_MeTrfase_PRMT5. 
Pfam
 PF05185; PRMT5 
SMART
  
PROSITE
  
PRINTS