UniProt Accession

 MEP50_HUMAN; Q9BQA1; B3KMW6; Q3LID2; Q53FU2; Q6JZZ5; Q96GK4; Q9BWY3 

Genbank Protein ID

 AF478464; AK022860; AK223189; AY225316; AB073603; AL390195; CH471122; BC001679; BC006477; BC009411; BC011778; BC016946; AB074171 

Genbank Nucleotide ID

 AAL79917.1; BAG51128.1; BAD96909.1; AAP79114.1; BAD38641.1; CAC36041.1; EAW56493.1; AAH01679.1; AAH06477.1; AAH09411.1; AAH11778.1; AAH16946.1; BAE45736.1 

Protein Name

 Methylosome protein 50 

Protein Synonyms/Alias

 MEP-50; Androgen receptor cofactor p44; WD repeat-containing protein 77; p44/Mep50 

Gene Name

 WDR77 

Gene Synonyms/Alias

 MEP50; HKMT1069; Nbla10071 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
3	*****MRKETPPPLV	ubiquitination	[1]
121	LIVSKFCKYEHDDIV	ubiquitination	[1, 2]
145	TQAVSGSKDICIKVW	ubiquitination	[1]
179	CVAASPHKDSVFLSC	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. 

Sequence Annotation

 REPEAT 22 75 WD 1.
 REPEAT 78 116 WD 2.
 REPEAT 123 162 WD 3.
 REPEAT 165 205 WD 4.
 REPEAT 209 250 WD 5.
 REPEAT 253 293 WD 6.
 REPEAT 295 330 WD 7.
 MOD_RES 5 5 Phosphothreonine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 342 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; IGI:MGI.
 GO:0034660; P:ncRNA metabolic process; TAS:Reactome.
 GO:0060770; P:negative regulation of epithelial cell proliferation involved in prostate gland development; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0060528; P:secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development; IEA:Compara.
 GO:0000387; P:spliceosomal snRNP assembly; TAS:Reactome. 

Interpro

 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS