UniProt Accession

 XRCC4_HUMAN; Q13426; A8K3X4; Q9BS72; Q9UP94 

Genbank Protein ID

 U40622; AF055285; AF055279; AF055280; AF055281; AF055282; AF055283; AF055284; AF055285; AF055279; AF055280; AF055281; AF055282; AF055283; AF055284; AB017445; BT007216; AK290739; AY940097; CH471084; BC005259; BC016314 

Genbank Nucleotide ID

 AAC50339.1; AAD47297.1; AAD47297.1; AAD47297.1; AAD47297.1; AAD47297.1; AAD47297.1; AAD47297.1; AAD47298.1; AAD47298.1; AAD47298.1; AAD47298.1; AAD47298.1; AAD47298.1; AAD47298.1; BAB20668.1; AAP35880.1; BAF83428.1; AAX14046.1; EAW95898.1; AAH05259.1; AAH16314.1 

Protein Name

 DNA repair protein XRCC4 

Protein Synonyms/Alias

 X-ray repair cross-complementing protein 4 

Gene Name

 XRCC4 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
102	FFFEKNLKDVSFRLG	ubiquitination	[1]
146	AKNEHLQKENERLLR	ubiquitination	[1]
178	ALETDLYKRFILVLN	ubiquitination	[1, 2, 3]
188	ILVLNEKKTKIRSLH	ubiquitination	[2]
197	KIRSLHNKLLNAAQE	ubiquitination	[1, 4, 5]
210	QEREKDIKQEGETAI	sumoylation	[6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] SUMO modification of human XRCC4 regulates its localization and function in DNA double-strand break repair.
 Yurchenko V, Xue Z, Sadofsky MJ.
 Mol Cell Biol. 2006 Mar;26(5):1786-94. [PMID: 16478998] 

Functional Description

 Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. 

Sequence Annotation

 REGION 180 213 Interacts with LIG4.
 MOD_RES 232 232 Phosphoserine (By similarity).
 MOD_RES 233 233 Phosphothreonine (By similarity).
 MOD_RES 256 256 Phosphoserine.
 MOD_RES 260 260 Phosphoserine; by PRKDC.
 MOD_RES 320 320 Phosphoserine; by PRKDC.
 MOD_RES 327 327 Phosphoserine.
 MOD_RES 328 328 Phosphoserine.
 CROSSLNK 210 210 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Alternative splicing; Coiled coil; Complete proteome; DNA damage; DNA recombination; DNA repair; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 336 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0032807; C:DNA ligase IV complex; IDA:UniProtKB.
 GO:0005958; C:DNA-dependent protein kinase-DNA ligase 4 complex; IDA:MGI.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0071285; P:cellular response to lithium ion; IEA:Compara.
 GO:0007417; P:central nervous system development; IEA:Compara.
 GO:0051103; P:DNA ligation involved in DNA repair; IDA:UniProtKB.
 GO:0006303; P:double-strand break repair via nonhomologous end joining; IDA:UniProtKB.
 GO:0075713; P:establishment of integrated proviral latency; TAS:Reactome.
 GO:0033152; P:immunoglobulin V(D)J recombination; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0045190; P:isotype switching; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0048146; P:positive regulation of fibroblast proliferation; IEA:Compara.
 GO:0051351; P:positive regulation of ligase activity; IDA:UniProtKB.
 GO:0050769; P:positive regulation of neurogenesis; IEA:Compara.
 GO:0002328; P:pro-B cell differentiation; IEA:Compara.
 GO:0010332; P:response to gamma radiation; IEA:Compara.
 GO:0010165; P:response to X-ray; IDA:UniProtKB.
 GO:0033077; P:T cell differentiation in thymus; IEA:Compara. 

Interpro

 IPR010585; DNA_repair_prot_XRCC4.
 IPR014751; XRCC4_C.
 IPR009089; XRCC4_N. 

Pfam

 PF06632; XRCC4 

SMART

  

PROSITE

  

PRINTS