CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016424
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoenolpyruvate carboxykinase [GTP], mitochondrial 
Protein Synonyms/Alias
 PEPCK-M; Phosphoenolpyruvate carboxylase 
Gene Name
 Pck2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
90IRKLPKYKNCWLARTacetylation[1, 2]
90IRKLPKYKNCWLARTsuccinylation[2]
108DVARVESKTVIVTPSacetylation[2]
108DVARVESKTVIVTPSsuccinylation[2]
262GNSLLGKKCFALRIAphosphoglycerylation[3]
322RPALPGWKVECVGDDacetylation[1]
410SWLGKPWKPGDKEPCacetylation[1]
414KPWKPGDKEPCAHPNacetylation[1]
457IFGGRRPKGVPLVYEacetylation[2]
457IFGGRRPKGVPLVYEsuccinylation[2]
491AAAEHKGKTIMHDPFacetylation[2]
491AAAEHKGKTIMHDPFsuccinylation[2]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 
Functional Description
 Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (By similarity). 
Sequence Annotation
 NP_BIND 305 310 GTP (By similarity).
 NP_BIND 548 551 GTP (By similarity).
 REGION 421 423 Substrate binding (By similarity).
 ACT_SITE 306 306 By similarity.
 METAL 262 262 Manganese (By similarity).
 METAL 282 282 Manganese (By similarity).
 METAL 329 329 Manganese (By similarity).
 BINDING 104 104 Substrate (By similarity).
 BINDING 255 255 Substrate; via amide nitrogen (By
 BINDING 262 262 Substrate (By similarity).
 BINDING 304 304 Substrate (By similarity).
 BINDING 423 423 GTP (By similarity).
 BINDING 454 454 GTP (By similarity).  
Keyword
 Complete proteome; Decarboxylase; Gluconeogenesis; GTP-binding; Lyase; Manganese; Metal-binding; Mitochondrion; Nucleotide-binding; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 640 AA 
Protein Sequence
MAAMYLPGLR LSRHGLRPWC WSPCRSIQTL HVLSGDMSQL PAGVRDFVAR SAHLCQPEGI 60
HICDGTEAEN TAILALLEEQ GLIRKLPKYK NCWLARTDPK DVARVESKTV IVTPSQRDTV 120
PLLAGGARGQ LGNWMSPDEF QRAVDERFPG CMQGRIMYVL PFSMGPVGSP LSRIGVQLTD 180
SAYVVASMRI MTRLGTPVLQ ALGDGDFIKC LHSVGQPLTG HGDPVGQWPC NPEKTLIGHV 240
PDQREIVSFG SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITN PAGKKRYVAA 300
AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGQ LRAINPENGF FGVAPGTSAA 360
TNPNAMATIQ SNTLFTNVAE TSDGGVYWEG IDQPLPPGVT ITSWLGKPWK PGDKEPCAHP 420
NSRFCVPARQ CPIMDPAWEA PEGVPIDAII FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS 480
ESTAAAEHKG KTIMHDPFAM RPFFGYNFGR YLEHWLSMEG QKGARLPRIF HVNWFRRDEA 540
GRFLWPGFGE NARVLDWICR RLEGEDSAQE TPIGLVPKEG ALDLSGLSAV DTSQLFSIPK 600
DFWEQEVRDI RGYLTEQVNQ DLPKEVLAEL EALEGRVQKM 640 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:EC.
 GO:0006094; P:gluconeogenesis; IEA:UniProtKB-UniPathway. 
Interpro
 IPR018091; PEP_carboxykin_GTP_CS.
 IPR013035; PEP_carboxykinase_C.
 IPR008209; PEP_carboxykinase_GTP.
 IPR008210; PEP_carboxykinase_N. 
Pfam
 PF00821; PEPCK 
SMART
  
PROSITE
 PS00505; PEPCK_GTP 
PRINTS