CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012183
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Four and a half LIM domains protein 3 
Protein Synonyms/Alias
 FHL-3; Skeletal muscle LIM-protein 2; SLIM-2 
Gene Name
 FHL3 
Gene Synonyms/Alias
 SLIM2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
157CVPCYENKFAPRCARubiquitination[1, 2]
167PRCARCSKTLTQGGVubiquitination[2, 3, 4, 5, 6, 7, 8]
235IVGLGGGKYVSFEDRubiquitination[2, 3, 7]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 DOMAIN 40 92 LIM zinc-binding 1.
 DOMAIN 101 153 LIM zinc-binding 2.
 DOMAIN 162 212 LIM zinc-binding 3.
 DOMAIN 221 275 LIM zinc-binding 4.
 ZN_FING 7 31 C4-type (Potential).  
Keyword
 3D-structure; Complete proteome; LIM domain; Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 280 AA 
Protein Sequence
MSESFDCAKC NESLYGRKYI QTDSGPYCVP CYDNTFANTC AECQQLIGHD SRELFYEDRH 60
FHEGCFRCCR CQRSLADEPF TCQDSELLCN DCYCSAFSSQ CSACGETVMP GSRKLEYGGQ 120
TWHEHCFLCS GCEQPLGSRS FVPDKGAHYC VPCYENKFAP RCARCSKTLT QGGVTYRDQP 180
WHRECLVCTG CQTPLAGQQF TSRDEDPYCV ACFGELFAPK CSSCKRPIVG LGGGKYVSFE 240
DRHWHHNCFS CARCSTSLVG QGFVPDGDQV LCQGCSQAGP 280 
Gene Ontology
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0001725; C:stress fiber; IEA:Compara.
 GO:0030018; C:Z disc; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0030036; P:actin cytoskeleton organization; IEA:Compara.
 GO:0007517; P:muscle organ development; TAS:ProtInc. 
Interpro
 IPR001781; Znf_LIM. 
Pfam
 PF00412; LIM 
SMART
 SM00132; LIM 
PROSITE
 PS00478; LIM_DOMAIN_1
 PS50023; LIM_DOMAIN_2 
PRINTS