CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011724
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Polypeptide N-acetylgalactosaminyltransferase 1 
Protein Synonyms/Alias
 Polypeptide GalNAc transferase 1; GalNAc-T1; pp-GaNTase 1; Protein-UDP acetylgalactosaminyltransferase 1; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1; Polypeptide N-acetylgalactosaminyltransferase 1 soluble form 
Gene Name
 GALNT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
172RPLESYVKKLKVPVHubiquitination[1]
175ESYVKKLKVPVHVIRubiquitination[2]
390IISPGVTKVDYGDISubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b and Muc7. 
Sequence Annotation
 DOMAIN 429 551 Ricin B-type lectin.
 REGION 115 225 Catalytic subdomain A.
 REGION 285 347 Catalytic subdomain B.
 CARBOHYD 95 95 N-linked (GlcNAc...) (Potential).
 CARBOHYD 552 552 N-linked (GlcNAc...) (Potential).
 DISULFID 106 339 By similarity.
 DISULFID 330 408 By similarity.
 DISULFID 442 459 By similarity.
 DISULFID 482 497 By similarity.
 DISULFID 523 540 By similarity.  
Keyword
 Alternative splicing; Calcium; Complete proteome; Disulfide bond; Glycoprotein; Glycosyltransferase; Golgi apparatus; Lectin; Manganese; Membrane; Polymorphism; Reference proteome; Secreted; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 559 AA 
Protein Sequence
MRKFAYCKVV LATSLIWVLL DMFLLLYFSE CNKCDEKKER GLPAGDVLEP VQKPHEGPGE 60
MGKPVVIPKE DQEKMKEMFK INQFNLMASE MIALNRSLPD VRLEGCKTKV YPDNLPTTSV 120
VIVFHNEAWS TLLRTVHSVI NRSPRHMIEE IVLVDDASER DFLKRPLESY VKKLKVPVHV 180
IRMEQRSGLI RARLKGAAVS KGQVITFLDA HCECTVGWLE PLLARIKHDR RTVVCPIIDV 240
ISDDTFEYMA GSDMTYGGFN WKLNFRWYPV PQREMDRRKG DRTLPVRTPT MAGGLFSIDR 300
DYFQEIGTYD AGMDIWGGEN LEISFRIWQC GGTLEIVTCS HVGHVFRKAT PYTFPGGTGQ 360
IINKNNRRLA EVWMDEFKNF FYIISPGVTK VDYGDISSRV GLRHKLQCKP FSWYLENIYP 420
DSQIPRHYFS LGEIRNVETN QCLDNMARKE NEKVGIFNCH GMGGNQVFSY TANKEIRTDD 480
LCLDVSKLNG PVTMLKCHHL KGNQLWEYDP VKLTLQHVNS NQCLDKATEE DSQVPSIRDC 540
NGSRSQQWLL RNVTLPEIF 559 
Gene Ontology
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0030145; F:manganese ion binding; IDA:BHF-UCL.
 GO:0004653; F:polypeptide N-acetylgalactosaminyltransferase activity; IDA:BHF-UCL.
 GO:0016266; P:O-glycan processing; TAS:Reactome.
 GO:0043687; P:post-translational protein modification; TAS:Reactome.
 GO:0018242; P:protein O-linked glycosylation via serine; IDA:BHF-UCL.
 GO:0018243; P:protein O-linked glycosylation via threonine; IDA:BHF-UCL. 
Interpro
 IPR001173; Glyco_trans_2.
 IPR000772; Ricin_B_lectin. 
Pfam
 PF00535; Glycos_transf_2
 PF00652; Ricin_B_lectin 
SMART
 SM00458; RICIN 
PROSITE
 PS50231; RICIN_B_LECTIN 
PRINTS