CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018862
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho guanine nucleotide exchange factor 1 
Protein Synonyms/Alias
 115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5 
Gene Name
 ARHGEF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
278DEPAKTKKGLSSILDubiquitination[1]
303VPDFRHLKAEVDAEKubiquitination[1]
427DTLHSLPKSQVKRQEubiquitination[1]
525AEGSWFQKISSRFCSubiquitination[1, 2, 3]
570RCRRLQLKDMIPTEMubiquitination[1]
582TEMQRLTKYPLLLQSubiquitination[1, 2]
602EEPTEREKVELAAECubiquitination[1]
654DPMLSEFKNLDITKKubiquitination[1]
660FKNLDITKKKLVHEGubiquitination[1]
661KNLDITKKKLVHEGPubiquitination[1]
662NLDITKKKLVHEGPLubiquitination[1]
703QDERLLLKSHSRTLTubiquitination[1]
716LTPTPDGKTMLRPVLubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. 
Sequence Annotation
 DOMAIN 41 232 RGSL.
 DOMAIN 416 605 DH.
 DOMAIN 647 760 PH.
 MOD_RES 738 738 Phosphotyrosine; by JAK2.
 MOD_RES 863 863 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; GTPase activation; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 912 AA 
Protein Sequence
MASLSTWSSP AEPREMEDFA RGAASPGPSR PGLVPVSIIG AEDEDFENEL ETNSEEQNSQ 60
FQSLEQVKRR PAHLMALLQH VALQFEPGPL LCCLHADMLG SLGPKEAKKA FLDFYHSFLE 120
KTAVLRVPVP PNVAFELDRT RADLISEDVQ RRFVQEVVQS QQVAVGRQLE DFRSKRLMGM 180
TPWEQELAQL EAWVGRDRAS YEARERHVAE RLLMHLEEMQ HTISTDEEKS AAVVNAIGLY 240
MRHLGVRTKS GDKKSGRNFF RKKVMGNRRS DEPAKTKKGL SSILDAARWN RGEPQVPDFR 300
HLKAEVDAEK PGATDRKGGV GMPSRDRNIG APGQDTPGVS LHPLSLDSPD REPGADAPLE 360
LGDSSPQGPM SLESLAPPES TDEGAETESP EPGDEGEPGR SGLELEPEEP PGWRELVPPD 420
TLHSLPKSQV KRQEVISELL VTEAAHVRML RVLHDLFFQP MAECLFFPLE ELQNIFPSLD 480
ELIEVHSLFL DRLMKRRQES GYLIEEIGDV LLARFDGAEG SWFQKISSRF CSRQSFALEQ 540
LKAKQRKDPR FCAFVQEAES RPRCRRLQLK DMIPTEMQRL TKYPLLLQSI GQNTEEPTER 600
EKVELAAECC REILHHVNQA VRDMEDLLRL KDYQRRLDLS HLRQSSDPML SEFKNLDITK 660
KKLVHEGPLT WRVTKDKAVE VHVLLLDDLL LLLQRQDERL LLKSHSRTLT PTPDGKTMLR 720
PVLRLTSAMT REVATDHKAF YVLFTWDQEA QIYELVAQTV SERKNWCALI TETAGSLKVP 780
APASRPKPRP SPSSTREPLL SSSENGNGGR ETSPADARTE RILSDLLPFC RPGPEGQLAA 840
TALRKVLSLK QLLFPAEEDN GAGPPRDGDG VPGGGPLSPA RTQEIQENLL SLEETMKQLE 900
ELEEEFCRLR PLLSQLGGNS VPQPGCT 927 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; TAS:ProtInc.
 GO:0008283; P:cell proliferation; TAS:ProtInc.
 GO:0050771; P:negative regulation of axonogenesis; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0007266; P:Rho protein signal transduction; TAS:ProtInc.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000219; DH-domain.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015212; Regulat_G_prot_signal-like.
 IPR016137; Regulat_G_prot_signal_superfam.
 IPR015721; RhoGEF-like. 
Pfam
 PF09128; RGS-like
 PF00621; RhoGEF 
SMART
 SM00233; PH
 SM00325; RhoGEF 
PROSITE
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN 
PRINTS