CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039928
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Runt-related transcription factor 3 
Protein Synonyms/Alias
  
Gene Name
 RUNX3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
36RNASAVMKNQVARFNubiquitination[1, 2, 3, 4]
93EPRRHRQKLEDQTKPubiquitination[4]
99QKLEDQTKPFPDRFGacetylation[4, 5]
99QKLEDQTKPFPDRFGubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 322 AA 
Protein Sequence
MVVALGDVPD GTVVTVMAGN DENYSAELRN ASAVMKNQVA RFNDLRFVGR SGRGKSFTLT 60
ITVFTNPTQV ATYHRAIKVT VDGPREPRRH RQKLEDQTKP FPDRFGDLER LRMRVTPSTP 120
SPRGSLSTTS HFSSQPQTPI QGTSELNPFS DPRQFDRSFP TLPTLTESRF PDPRMHYPGA 180
MSAAFPYSAT PSGTSISSLS VAGMPATSRF HHTYLPPPYP GAPQNQSGPF QANPSPYHLY 240
YGTSSGSYQF SMVAGSSSGG DRSPTRMLAS CTSSAASVAA GNLMNPSLGG QSDGVEADGS 300
HSNSPTALST PGRMDEAVWR PY 322 
Gene Ontology
 GO:0005634; C:nucleus; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro. 
Interpro
 IPR000040; AML1_Runt.
 IPR008967; p53-like_TF_DNA-bd.
 IPR012346; p53/RUNT-type_TF_DNA-bd.
 IPR013524; Runt_dom.
 IPR027384; Runx_central_dom.
 IPR013711; RunxI_C_dom. 
Pfam
 PF00853; Runt
 PF08504; RunxI 
SMART
  
PROSITE
 PS51062; RUNT 
PRINTS
 PR00967; ONCOGENEAML1.