CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023808
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 5'-AMP-activated protein kinase subunit beta-1 
Protein Synonyms/Alias
 AMPK subunit beta-1; AMPKb 
Gene Name
 PRKAB1 
Gene Synonyms/Alias
 AMPK 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
57EEIKAPEKEEFLAWQubiquitination[1, 2]
89FRWTGGGKEVYLSGSubiquitination[3]
102GSFNNWSKLPLTRSHubiquitination[3, 4]
157NNIIQVKKTDFEVFDubiquitination[3]
195HQEPYVCKPEERFRAubiquitination[3, 5]
268YVTTLLYKPI*****ubiquitination[3, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). 
Sequence Annotation
 REGION 68 163 Glycogen-binding domain (By similarity).
 MOD_RES 4 4 Phosphothreonine.
 MOD_RES 5 5 Phosphoserine.
 MOD_RES 6 6 Phosphoserine.
 MOD_RES 19 19 Phosphothreonine.
 MOD_RES 24 24 Phosphoserine; by autocatalysis (By
 MOD_RES 25 25 Phosphoserine; by autocatalysis (By
 MOD_RES 40 40 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 101 101 Phosphoserine (By similarity).
 MOD_RES 108 108 Phosphoserine.
 MOD_RES 148 148 Phosphothreonine.
 MOD_RES 182 182 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine.  
Keyword
 Complete proteome; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Lipoprotein; Myristate; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 270 AA 
Protein Sequence
MGNTSSERAA LERHGGHKTP RRDSSGGTKD GDRPKILMDS PEDADLFHSE EIKAPEKEEF 60
LAWQHDLEVN DKAPAQARPT VFRWTGGGKE VYLSGSFNNW SKLPLTRSHN NFVAILDLPE 120
GEHQYKFFVD GQWTHDPSEP IVTSQLGTVN NIIQVKKTDF EVFDALMVDS QKCSDVSELS 180
SSPPGPYHQE PYVCKPEERF RAPPILPPHL LQVILNKDTG ISCDPALLPE PNHVMLNHLY 240
ALSIKDGVMV LSATHRYKKK YVTTLLYKPI 270 
Gene Ontology
 GO:0031588; C:AMP-activated protein kinase complex; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004672; F:protein kinase activity; IDA:UniProtKB.
 GO:0007050; P:cell cycle arrest; TAS:Reactome.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
 GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
 GO:0010628; P:positive regulation of gene expression; IDA:UniProtKB.
 GO:0051291; P:protein heterooligomerization; IEA:Compara.
 GO:0050790; P:regulation of catalytic activity; IEA:Compara. 
Interpro
 IPR006828; AMP_prot_kin_bsu_interact-dom. 
Pfam
 PF04739; AMPKBI 
SMART
 SM01010; AMPKBI 
PROSITE
  
PRINTS