UniProt Accession

 CHCH3_MOUSE; Q9CRB9 

Genbank Protein ID

 AK002263; AK003519; BC021941 

Genbank Nucleotide ID

 BAB21974.1; BAB22832.1; AAH21941.1 

Protein Name

 Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 Chchd3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
63	SVSDEDLKRRVAEEL	ubiquitination	[1]
130	LARQLEEKDRVMRKQ	acetylation	[2]
142	RKQDAFYKEQLARLE	acetylation	[2]
157	ERSSEFYKVTTEEYQ	acetylation	[2]
157	ERSSEFYKVTTEEYQ	ubiquitination	[1]
165	VTTEEYQKAAEEVEA	acetylation	[2]
173	AAEEVEAKFKRYEYH	ubiquitination	[1]
189	VCADLQTKILQCYRQ	acetylation	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Required for maintenance of mitochondrial crista integrity and mitochondrial function. May act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. 

Sequence Annotation

 DOMAIN 176 217 CHCH.
 MOD_RES 49 49 Phosphotyrosine (By similarity).
 MOD_RES 50 50 Phosphoserine (By similarity).
 MOD_RES 58 58 Phosphoserine.
 MOD_RES 142 142 N6-acetyllysine (By similarity).
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Lipoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Myristate; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0019902; F:phosphatase binding; ISS:UniProtKB.
 GO:0032947; F:protein complex scaffold; IMP:UniProtKB.
 GO:0001227; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; ISS:UniProtKB.
 GO:0007007; P:inner mitochondrial membrane organization; IEA:Compara.
 GO:0008053; P:mitochondrial fusion; IEA:Compara. 

Interpro

 IPR007964; DUF737. 

Pfam

 PF05300; DUF737 

SMART

  

PROSITE

  

PRINTS