CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-042345
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Histone lysine demethylase PHF8 
Protein Synonyms/Alias
  
Gene Name
 PHF8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
34VPILVLKKDGLGMTLubiquitination[1]
59EHYVGSDKEIDVIDVubiquitination[1]
75RQADCKMKLGDFVKYubiquitination[1]
81MKLGDFVKYYYSGKRubiquitination[1]
87VKYYYSGKREKVLNVacetylation[2]
87VKYYYSGKREKVLNVubiquitination[1]
314AFRAWTRKEALPDHEubiquitination[1, 2]
335VRTVQLIKDLAREIRubiquitination[1]
354IFQQNVGKTSNIFGLubiquitination[1, 2, 3, 4]
391SRLSLPSKNGSKKKGubiquitination[1]
396PSKNGSKKKGLKPKEubiquitination[1]
533DERLGKEKATLIIRPubiquitination[1]
600GGILDLLKASRQVGGubiquitination[1, 4]
783GLAAAAAKLAQQELQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 953 AA 
Protein Sequence
XSSDEVILKP TGNQLTVEFL EENSFSVPIL VLKKDGLGMT LPSPSFTVRD VEHYVGSDKE 60
IDVIDVTRQA DCKMKLGDFV KYYYSGKREK VLNVISLEFS DTRLSNLVET PKIVRKLSWV 120
ENLWPEECVF ERPNVQKYCL MSVRDSYTDF HIDFGGTSVW YHVLKGEKIF YLIRPTNANL 180
TLFECWSSSS NQNEMFFGDQ VDKCYKCSVK QGQTLFIPTG WIHAVLTPVD CLAFGGNFLH 240
SLNIEMQLKA YEIEKRLSTA DLFRFPNFET ICWYVGKHIL DIFRGLRENR RHPASYLVHG 300
GKALNLAFRA WTRKEALPDH EDEIPETVRT VQLIKDLARE IRLVEDIFQQ NVGKTSNIFG 360
LQRIFPAGSI PLTRPAHSTS VSMSRLSLPS KNGSKKKGLK PKELFKKAER KGKESSALGP 420
AGQLSYNLMD TYSHQALKTG SFQKAKFNIT GACLNDSDDD SPDLDLDGNE SPLALLMSNG 480
STKRVKSLSK SRRTKIAKKV DKARLMAEQV MEDEFDLDSD DELQIDERLG KEKATLIIRP 540
KFPRKLPRAK PCSDPNRVRE PGEVEFDIEE DYTTDEDMVE GVEGKLGNGS GAGGILDLLK 600
ASRQVGGPDY AALTEAPASP STQEAIQGML CMANLQSSSS SPATSSLQAW WTGGQDRSSG 660
SSSSGLGTVS NSPASQRTPG KRPIKRPAYW RTESEEEEEN ASLDEQDSLG ACFKDAEYIY 720
PSLESDDDDP ALKSRPKKKK NSDDAPWSPK ARVTPTLPKQ DRPVREGTRV ASIETGLAAA 780
AAKLAQQELQ KAQKKKYIKK KPLLKEVEQP RPQDSNLSLT VPAPTVAATP QLVTSSSPLP 840
PPEPKQEALS GSLADHEYTA RPNAFGMAQA NRSTTPMAPG VFLTQRRPSV GSQSNQAGQG 900
KRPKKGLATA KQRLGRILKI HRNGKLLLRQ VIVQAECRQA IHEPKLKRRD AHP 953 
Gene Ontology
  
Interpro
 IPR003347; JmjC_dom. 
Pfam
 PF02373; JmjC 
SMART
 SM00558; JmjC 
PROSITE
 PS51184; JMJC 
PRINTS