CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024777
UniProt Accession
Genbank Protein ID
  
Genbank Nucleotide ID
  
Protein Name
 U5 small nuclear ribonucleoprotein 200 kDa helicase 
Protein Synonyms/Alias
 BRR2 homolog; U5 snRNP-specific 200 kDa protein; U5-200KD 
Gene Name
 Snrnp200 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
944GISHDDLKGDPLLDQacetylation[1]
1176KMGKTIHKYVHLFPKacetylation[1]
1718GSKKDFFKKFLYEPLacetylation[1]
2029LSYEVVDKDSIRSGGacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome (By similarity). 
Sequence Annotation
 DOMAIN 490 673 Helicase ATP-binding 1.
 DOMAIN 684 921 Helicase C-terminal 1.
 DOMAIN 982 1289 SEC63 1.
 DOMAIN 1340 1515 Helicase ATP-binding 2.
 DOMAIN 1548 1756 Helicase C-terminal 2.
 DOMAIN 1815 2127 SEC63 2.
 NP_BIND 503 510 ATP (By similarity).
 NP_BIND 1353 1360 ATP (By similarity).
 MOTIF 615 618 DEAH box.
 MOTIF 1457 1460 DEAH box.
 MOD_RES 26 26 Phosphoserine (By similarity).
 MOD_RES 225 225 Phosphoserine (By similarity).
 MOD_RES 709 709 Phosphotyrosine.
 MOD_RES 971 971 N6-acetyllysine; alternate (By
 MOD_RES 2134 2134 Phosphothreonine (By similarity).
 MOD_RES 2136 2136 Phosphoserine (By similarity).
 MOD_RES 2138 2138 Phosphoserine (By similarity).
 CROSSLNK 944 944 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 971 971 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1071 1071 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 1199 1199 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 2094 2094 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; ATP-binding; Coiled coil; Complete proteome; Helicase; Hydrolase; Isopeptide bond; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; Spliceosome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2139 AA 
Protein Sequence
MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK 60
PQMQEERRAK RRKRDEDRHD INKMKGYTLL SEGIDEMVGI IYKPKTKETR ETYEVLLSFI 120
QAALGDQPRD ILCGAADEVL AVLKNEKLRD KERRREIDLL LGQTDDTRYH VLVNLGKKIT 180
DYGGDKEIQN MDDNIDETYG VNVQFESDEE EGDEDVYGEV REEASDDDME GDEAVVRCTL 240
SANLVASGEL MSSKKKDLHP RDIDAFWLQR QLSRFYDDAI VSQKKADEVL EILKTASDDR 300
ECENQLVLLL GFNTFDFIKV LRQHRMMILY CTLLASAQSE AEKERIMGKM EADPELSKFL 360
YQLHETEKED LIREERSRRE RVRQSRMDTD LETMDLDQGG EALAPRQVLD LEDLVFTQGS 420
HFMANKRCQL PDGSFRRQRK GYEEVHVPAL KPKPFGSEEQ LLPVEKLPKY AQAGFEGFKT 480
LNRIQSKLYR AALETDENLL LCAPTGAGKT NVALMCMLRE IGKHINMDGT INVDDFKIIY 540
IAPMRSLVQE MVGSFGKRLA TYGITVAELT GDHQLCKEEI SATQIIVCTP EKWDIITRKG 600
GERTYTQLVR LIVLDEIHLL HDDRGPVLEA LVARAIRNIE MTQEDVRLIG LSATLPNYED 660
VATFLRVDPA KGLFYFDNSF RPVPLEQTYV GITEKKAIKR FQIMNEIVYE KIMEHAGKNQ 720
VLVFVHSRKE TGKTARAIRD MCLEKDTLGL FLREGSASTE VLRTEAEQCK NLELKDLLPY 780
GFAIHHAGMT RVDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR 840
WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPIE SQMVSKLPDM 900
LNAEIVLGNV QNAKDAVNWL GYAYLYIRML RSPTLYGISH DDLKGDPLLD QRRLDLVHTA 960
ALMLDKNNLV KYDKKTGNFQ VTELGRIASH YYITNDTVQT YNQLLKPTLS EIELFRVFSL 1020
SSEFKNITVR EEEKLELQKL LERVPIPVKE SIEEPSAKIN VLLQAFISQL KLEGFALMAD 1080
MVYVTQSAGR LMRAIFEIVL NRGWAQLTDK TLNLCKMIDK RMWQSMCPLR QFRKLPEEVV 1140
KKIEKKNFPF ERLYDLNHNE IGELIRMPKM GKTIHKYVHL FPKLELSVHL QPITRSTLKV 1200
ELTITPDFQW DEKARLVHGS SEAFWILVED VDSEVILHHE YFLLKAKYAQ DEHLITFFVP 1260
VFEPLPPQYF IRVVSDRWLS CETQLPVSFR HLILPEKYPP PTELLDLQPL PVSALRNSAF 1320
ESLYQDKFPF FNPIQTQVFN TVYNSDDNVF VGAPTGSGKT ICAEFAILRM LLQNSEGRCV 1380
YITPMEALAE QVYMDWYEKF QDRLNKKVVL LTGETSTDLK LLGKGNIIIS TPEKWDILSR 1440
RWKQRKNVQN INLFVVDEVH LIGGENGPVL EVICSRMRYI SSQIERPIRI VALSSSLSNA 1500
KDVAHWLGCS ATSTFNFHPN VRPVPLELHI QGFNISHTQT RLLSMAKPVY HAITKHSPKK 1560
PVIVFVPSRK QTRLTAIDIL TTCAADIQRQ RFLHCTEKDL IPYLEKLSDS TLKETLLNGV 1620
GYLHEGLSPM ERRLVEQLFS SGAIQVVVAS RSLCWGMNVA AHLVIIMDTQ YYNGKIHAYV 1680
DYPIYDVLQM VGHANRPLQD DEGRCVIMCQ GSKKDFFKKF LYEPLPVESH LDHCMHDHFN 1740
AEIVTKTIEN KQDAVDYLTW TFLYRRMTQN PNYYNLQGIS HRHLSDHLSE LVEQTLSDLE 1800
QSKCISIEDE MDVAPLNLGM IAAYYYINYT TIELFSMSLN AKTKVRGLIE IISNAAEYEN 1860
IPIRHHEDNL LRQLAQKVPH KLNNPKFNDP HVKTNLLLQA HLSRMQLSAE LQSDTEEILS 1920
KAIRLIQACV DVLSSNGWLS PALAAMELAQ MVTQAMWSKD SYLKQLPHFT SEHIKRCTDK 1980
GVESVFDIME MEDEERNALL QLTDSQIADV ARFCNRYPNI ELSYEVVDKD SIRSGGPVVV 2040
LVQLEREEEV TGPVIAPLFP QKREEGWWVV IGDAKSNSLI SIKRLTLQQK AKVKLDFVAP 2100
ATGGHNYTLY FMSDAYMGCD QEYKFSVDVK EAETDSDSD 2139 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005682; C:U5 snRNP; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; ISS:UniProtKB.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR004179; Sec63-dom. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C
 PF02889; Sec63 
SMART
 SM00382; AAA
 SM00487; DEXDc
 SM00490; HELICc
 SM00611; SEC63
 SM00973; Sec63 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS