CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008055
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Homocitrate synthase, cytosolic isozyme 
Protein Synonyms/Alias
  
Gene Name
 LYS20 
Gene Synonyms/Alias
 YDL182W; D1298 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
127LRQYSHGKDMNYIAKacetylation[1]
174NIYKTVDKIGVNRVGacetylation[1]
331DPHDFGMKRYIHFANacetylation[1]
331DPHDFGMKRYIHFANubiquitination[2]
363NLTDDQIKEVTAKIKacetylation[1]
422LATIPAAKRTKPSA*acetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 MOD_RES 385 385 Phosphoserine.
 MOD_RES 396 396 Phosphothreonine.
 MOD_RES 401 401 Phosphoserine.
 MOD_RES 410 410 Phosphoserine.  
Keyword
 Amino-acid biosynthesis; Complete proteome; Cytoplasm; Lysine biosynthesis; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 428 AA 
Protein Sequence
MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA RALDDFGVDY 60
IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK VAVETGVDGV DVVIGTSKFL 120
RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV 180
GIADTVGCAN PRQVYELIRT LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL 240
GIGERNGITP LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC 300
AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV DQLNLNLTDD 360
QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL SAKKNKKNDS DVPELATIPA 420
AKRTKPSA 428 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0004410; F:homocitrate synthase activity; IDA:SGD.
 GO:0019878; P:lysine biosynthetic process via aminoadipic acid; IGI:SGD. 
Interpro
 IPR002034; AIPM/Hcit_synth_CS.
 IPR013785; Aldolase_TIM.
 IPR011872; Homocitrate_synth_fun/arc.
 IPR000891; PYR_CT. 
Pfam
 PF00682; HMGL-like 
SMART
  
PROSITE
 PS00815; AIPM_HOMOCIT_SYNTH_1
 PS00816; AIPM_HOMOCIT_SYNTH_2
 PS50991; PYR_CT 
PRINTS