UniProt Accession

 PRS4_YEAST; P40327; D6VRY1 

Genbank Protein ID

 X81070; L17040; Z48432; Z74055; BK006938 

Genbank Nucleotide ID

 CAA56957.1; AAA97498.1; CAA88352.1; CAA98563.1; DAA11841.1 

Protein Name

 26S protease regulatory subunit 4 homolog 

Protein Synonyms/Alias

 Tat-binding homolog 5 

Gene Name

 RPT2 

Gene Synonyms/Alias

 YHS4; YTA5; YDL007W; D2920 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
22	KGSNQKPKYEPPVQS	acetylation	[1]
30	YEPPVQSKFGRKKRK	acetylation	[1]
66	LLRMERIKDHLLLEE	ubiquitination	[2, 3]
175	VSVMKMDKSPTESYS	ubiquitination	[3]
229	YGAPGTGKTLLAKAV	ubiquitination	[3]
234	TGKTLLAKAVANQTS	ubiquitination	[2, 3]
255	VGSELIQKYLGDGPR	acetylation	[1]
255	VGSELIQKYLGDGPR	ubiquitination	[2, 3]
269	RLCRQIFKVAGENAP	ubiquitination	[3]
290	EIDAIGTKRYDSNSG	ubiquitination	[2, 3]
330	KVIMATNKIETLDPA	ubiquitination	[3]
347	RPGRIDRKILFENPD	ubiquitination	[3]
417	QVTAEDFKQAKERVM	ubiquitination	[3]
425	QAKERVMKNKVEENL	ubiquitination	[3]
427	KERVMKNKVEENLEG	ubiquitination	[3]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). Has ATPase activity. 

Sequence Annotation

 NP_BIND 223 230 ATP (Potential).
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Lipoprotein; Myristate; Nucleotide-binding; Nucleus; Proteasome; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 437 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0008540; C:proteasome regulatory particle, base subcomplex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016887; F:ATPase activity; IDA:SGD.
 GO:0043171; P:peptide catabolic process; IMP:SGD.
 GO:0045732; P:positive regulation of protein catabolic process; IDA:SGD.
 GO:0070682; P:proteasome regulatory particle assembly; IMP:SGD.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:SGD. 

Interpro

 IPR005937; 26S_Psome_P45.
 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00004; AAA 

SMART

 SM00382; AAA 

PROSITE

 PS00674; AAA 

PRINTS