CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044239
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Pre-mRNA-processing-splicing factor 8 
Protein Synonyms/Alias
  
Gene Name
 PRPF8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****XRIKIGLNSKMubiquitination[1, 2, 3]
10IKIGLNSKMPSRFPPubiquitination[1, 2, 3, 4, 5]
69RINTLFQKDRHTLAYmethylation[6]
69RINTLFQKDRHTLAYubiquitination[2]
78RHTLAYDKGWRVRTDubiquitination[1, 2, 3, 4, 7, 8]
87WRVRTDFKQYQVLKQubiquitination[1, 2, 3, 4, 7]
93FKQYQVLKQNPFWWTubiquitination[1, 2, 5, 7]
107THQRHDGKLWNLNNYubiquitination[2, 3, 4, 5, 7]
160FEESMKWKKLTNAQRmethylation[6, 9]
161EESMKWKKLTNAQRSmethylation[6, 9]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Mass spectrometry-based identification and characterisation of lysine and arginine methylation in the human proteome.
 Bremang M, Cuomo A, Agresta AM, Stugiewicz M, Spadotto V, Bonaldi T.
 Mol Biosyst. 2013 Jul 30;9(9):2231-47. [PMID: 23748837]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [9] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 250 AA 
Protein Sequence
XRIKIGLNSK MPSRFPPVVF YTPKELGGLG MLSMGHVLIP QSDLRRLTLE DLEDSWDRGI 60
PRINTLFQKD RHTLAYDKGW RVRTDFKQYQ VLKQNPFWWT HQRHDGKLWN LNNYRTDMIQ 120
ALGGVEGILE HTLFKGTYFP TWEGLFWEKA SGFEESMKWK KLTNAQRSGL NQIPNRRFTL 180
WWSPTINRAN VYVGFQVQLD LTGIFMHGKI PTLKISLIQI FRAHLWQKIH ESIVMDLCQV 240
FDQELDALEI 250 
Gene Ontology
  
Interpro
 IPR019581; Prp8_U5-snRNA-bd.
 IPR019580; Prp8_U6-snRNA-bd. 
Pfam
 PF10597; U5_2-snRNA_bdg
 PF10596; U6-snRNA_bdg 
SMART
  
PROSITE
  
PRINTS